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Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters

Applied Biochemistry and Biotechnology volume 176, pages 1071–1083 (2015)Cite this article

Abstract

Xylose-rich sawdust hydrolysate can be an economic substrate for the enzymatic production of xylitol, a specialty product. It is important to identify the process factors influencing xylitol production. This research aimed to screen the parameters significantly affecting bioxylitol synthesis from wood sawdust by xylose reductase (XR). Enzymatic bioxylitol production was conducted to estimate the effect of different variables reaction time (2–18 h), temperature (20–70 °C), pH (4.0–9.0), NADPH (1.17–5.32 g/L), and enzyme concentration (2–6 %) on the yield of xylitol. Fractional factorial design was followed to identify the key process factors. The screening design identified that time, temperature, and pH are the most significant factors influencing bioxylitol production among the variables with the values of 12 h, 35 °C, and 7.0, respectively. These conditions led to a xylitol yield of 71 % (w/w). This is the first report on the statistical screening of process variables influencing enzyme-based bioxylitol production from lignocellulosic biomass.

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Acknowledgments

The financial support for this research work from the Universiti Malaysia Pahang (UMP) and Ministry of Higher Education, Malaysia (MTUN-COE Research Grant No. RDU 121205) is gratefully acknowledged.

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Affiliations

  1. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, 26300, Kuantan, Pahang, Malaysia

    I. S. M. Rafiqul & A. M. M. Sakinah

  2. Faculty of Engineering Technology, Universiti Malaysia Pahang, 26300, Kuantan, Pahang, Malaysia

    A. W. Zularisam

Authors
  1. I. S. M. Rafiqul
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  2. A. M. M. Sakinah
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  3. A. W. Zularisam
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Correspondence to A. M. M. Sakinah.

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Rafiqul, I.S.M., Sakinah, A.M.M. & Zularisam, A.W. Enzymatic Production of Bioxylitol from Sawdust Hydrolysate: Screening of Process Parameters. Appl Biochem Biotechnol 176, 1071–1083 (2015). https://doi.org/10.1007/s12010-015-1630-2

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  • DOI: https://doi.org/10.1007/s12010-015-1630-2

Keywords

  • Bioconversion
  • Hemicellulosic hydrolysate
  • Screening design
  • Bioxylitol
  • Xylose
  • Xylose reductase