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Applied Biochemistry and Biotechnology

, Volume 176, Issue 3, pp 742–757 | Cite as

Cuscuta chinensis Ameliorates Immunosuppression and Urotoxic Effect of Cyclophosphamide by Regulating Cytokines - GM-CSF and TNF-Alpha

  • Nidhi Raju
  • Kunnathur Murugesan Sakthivel
  • Narayanan Kannan
  • Venugopal Vinod Prabhu
  • Chandrasekaran GuruvayoorappanEmail author
Article

Abstract

Cancer is the leading cause of death worldwide. Cyclophosphamide (CTX) is commonly used as anticancer drug which causes toxicity by its reactive metabolites such as acroline and phosphoramide mustard. In this study, Cuscuta chinensis (C. chinensis) (family: Convolvulaceae) was assessed for ability to restore mice against CTX-induced toxicity. Coadministration of C. chinensis extract (10 mg/kg BW, IP, daily) for ten consecutive days reduced CTX-induced (25 mg/kg BW, IP, daily) toxicity. Treatment with C. chinensis extract significantly (p < 0.01) increased the relative organ weight and body weight. Moreover, administration of C. chinensis extract significantly increased bone marrow cellulatity and α-esterase activity in CTX-treated mice which suggested its protective role on the hematopoietic system. The GSH content was drastically reduced by CTX administration in urinary bladder which was enhanced by treatment with C. chinensis extract, indicating that preventing acroline-mediated tissue damage or cell toxicity and also the extract decreased the urinary bladder nitric oxide (NO) level which proves recovery over urinary tract injury associated with CTX treatment. The administration of C. chinensis extract decreased serum urea, creatinine, and bilirubin levels when compared to CTX-alone-treated group. Histopathological analysis of the urinary bladder of CTX-alone-treated group showed necrotic damage whereas the C. chinensis-treated group showed normal bladder architecture. The above data clearly demonstrates chemoprotective role of C. chinensis against CTX-induced toxicities by regulating antioxidant and inflammatory mediators.

Keywords

Cyclophosphamide Cuscuta chinensis Chemoprotectant Antioxidants Inflammatory mediators 

Notes

Acknowledgments

The valuable support of Dr. Jannet Vennila, Director, School of Biotechnology and Health Sciences, Karunya University, is greatly acknowledged.

Conflict of Interest

The authors report no conflicts of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Nidhi Raju
    • 1
  • Kunnathur Murugesan Sakthivel
    • 1
  • Narayanan Kannan
    • 1
  • Venugopal Vinod Prabhu
    • 1
  • Chandrasekaran Guruvayoorappan
    • 1
    Email author
  1. 1.Department of BiotechnologyKarunya UniversityKarunya Nagar, CoimbatoreIndia

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