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In Vitro Antifungal Activity and Probable Fungicidal Mechanism of Aqueous Extract of Barleria Grandiflora

Applied Biochemistry and Biotechnology volume 175, pages 3571–3584 (2015)Cite this article

Abstract

Barleria grandiflora Dalz. (Acanthaceae) is being used in India to treat different types of disorders including skin infections. Therefore, there are good possibilities to find antifungal compounds in its extracts with novel mechanism of action. The main objectives of the present study were to evaluate the antifungal activity of plant extracts and to study its effects on metabolic pathways of A. fumigatus. The microbroth dilution assay was used to explore antifungal activity and MIC of various extracts. Metabolic profiles of control and treated cultures were collected from Q-TOF-MS interfaced with HPLC. Affected metabolic pathways of A. fumigatus after the treatment were analyzed by discrimination analysis of mass data. Antifungal activities were observed in hot and cold water extracts of the plant. Hot water extract of B. grandiflora showed significant activity against tested fungi in the range 0.625–1.25 mg/mL. Partial least discrimination analysis revealed that the hot water plant extract downregulated amino acid, glyoxylate pathway, and methylcitrate pathways at the same time due to the synergistic effects of secondary metabolites. Hot water extract also downregulated several other metabolic pathways unique to fungi indicating its specific activity toward fungi. B. grandiflora showed promising antifungal activity which can further be exploited by identification of active compounds, to inhibit the specific fungal pathways and development of novel therapeutic antifungal drugs.

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Affiliations

  1. Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, 124001, India

    Suman Kumari, Preeti Jain, Bhawana Sharma, Preeti Kadyan & Rajesh Dabur

  2. National Research Institute of Basic Ayurvedic Sciences, Central Council for Research in Ayurvedic Sciences, Nehru Garden, Kothrud, Pune, 411038, India

    Rajesh Dabur

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  1. Suman Kumari
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  2. Preeti Jain
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  3. Bhawana Sharma
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  4. Preeti Kadyan
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  5. Rajesh Dabur
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Correspondence to Rajesh Dabur.

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Kumari, S., Jain, P., Sharma, B. et al. In Vitro Antifungal Activity and Probable Fungicidal Mechanism of Aqueous Extract of Barleria Grandiflora . Appl Biochem Biotechnol 175, 3571–3584 (2015). https://doi.org/10.1007/s12010-015-1527-0

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  • DOI: https://doi.org/10.1007/s12010-015-1527-0

Keywords

  • Aspergillus fumigatus
  • Invasive aspergillosis
  • Barleria grandiflora Dalz
  • HPLC-Q-TOF-MS
  • Aminoadipate pathway
  • Glyoxylate pathway
  • Methylcitrate pathway