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Sustained Release of a Purified Tannin Component of Terminalia chebula from a Titanium Implant Surface Prevents Biofilm Formation by Staphylococcus aureus

Applied Biochemistry and Biotechnology volume 175, pages 3542–3556 (2015)Cite this article

Abstract

Although biofilms are formed on a variety of surfaces, of utmost significance are those formed on prosthetic devices used as implants. Such biofilms can lead to severe device-related infections that are difficult to treat. In a search for new antibiofilm agents that can be used as “active” implant coatings, purified fraction from a tannin-rich extract of Terminalia chebula was isolated and tested for its antibiofilm properties on a titanium implant material. The fraction, named as Fraction 7, was found to significantly reduce biofilm formation by hospital isolates of Staphylococcus aureus, at sub-inhibitory concentrations that were 64 times lower than the minimum inhibitory concentration (MIC). Simulated local delivery systems of the Fraction 7 set upon the surface of titanium alloy released the fraction in a controlled manner from a biodegradable carrier (PDLLA) and were found to significantly reduce biofilm formation by a methicillin-resistant hospital isolate of S. aureus in a load concentration dependent manner without preventing growth. This study therefore identifies a novel fraction from tannin-rich extract of T. chebula that has potential to be used as an antibiofilm coat on implant surfaces.

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Acknowledgements

Part of this work was done under the contingency grant allotted to the first author under the Faculty development program of University Grants Commission, Govt. of India vide letter F. No. 30-23/08(WRO). The authors also wish to thank Dr. Priti Mehta, Head, Department of Microbiology, KEM hospital, for providing the cultures, Mr. Ajay Pitre of Sushrut Surgicals for providing the titanium alloy pieces, and Dr. Arne Heydron, Denmark, for sending the COMSTAT 1 program.

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  1. Department of Microbiology, Ramnarain Ruia College, L.Napoo Road, Matunga, Mumbai, 400019, India

    Varsha Shukla

  2. Department of Microbiology, Bhavans College, Andheri (W), Mumbai, 400058, India

    Zarine Bhathena

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  1. Varsha Shukla
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  2. Zarine Bhathena
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Correspondence to Varsha Shukla.

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Shukla, V., Bhathena, Z. Sustained Release of a Purified Tannin Component of Terminalia chebula from a Titanium Implant Surface Prevents Biofilm Formation by Staphylococcus aureus . Appl Biochem Biotechnol 175, 3542–3556 (2015). https://doi.org/10.1007/s12010-015-1525-2

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  • DOI: https://doi.org/10.1007/s12010-015-1525-2

Keywords

  • Antibiofilm
  • Tannin
  • Titanium
  • Implant
  • Coated devices
  • MRSA