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MicroRNA-10b Triggers the Epithelial–Mesenchymal Transition (EMT) of Laryngeal Carcinoma Hep-2 Cells by Directly Targeting the E-cadherin

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Abstract

Laryngeal carcinoma is the second most common malignancy of the head and neck squamous cell carcinoma. Therefore, there is an urgent need to understand the molecular mechanism of its metastasis. The present study was designed to investigate effects of miR-10b on the invasion and migration of laryngeal Hep-2 cells. We found that miR-10b had limited effects on cell proliferation; however, it can significantly promote the migration and invasion of Hep-2 cells. Further studies revealed that overexpression of miR-10b can induce the epithelial–mesenchymal transition (EMT) of Hep-2 cells by acquiring mesenchymal spindle-like morphology and increasing the expression of N-cadherin (N-Cad) with a concomitant decrease of E-cadherin (E-Cad). However, the messenger RNA (mRNA) and protein level of transcription factors such as Snail, Slug, Twist and ZEB was not changed during this process. Bioinformatic analysis revealed that miR-10b can directly target CDH1 (E-Cad gene) at nucleotides 461 and 481 within the 3′-UTR. This was confirmed by the results that miR-10 downregulated the protein and mRNA levels of E-Cad via a time-dependent manner and luciferase analysis by use of four-nucleotide substitution in the core binding sites. The present study provided a better understanding of laryngeal carcinoma metastasis and the roles of miR-10b during this process.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (Grant No. 81172584).

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Authors and Affiliations

  1. Department of Orthopedics, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China

    Lin Zhang

  2. The Health Examination Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, 130051, China

    Jing Sun

  3. Department of Oncological Surgery, Tumor Hospital of Jilin Provice, 1018 Huguang Road, Changchun, Jilin Province, 130012, China

    Bin Wang

  4. Department of Oncology, Tumor Hospital of Jilin Provice, 1018 Huguang Road, Changchun, Jilin Province, 130012, China

    Ji-Chen Ren

  5. Department of Radiotherapy, Changchun Central Hospital, 1810 Renmin Street, Changchun, Jilin Province, 130012, China

    Wei Su

  6. Department of Radiotherapy, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China

    Tian Zhang

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  1. Lin Zhang
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  2. Jing Sun
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  3. Bin Wang
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  4. Ji-Chen Ren
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  5. Wei Su
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  6. Tian Zhang
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Correspondence to Tian Zhang.

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Zhang, L., Sun, J., Wang, B. et al. MicroRNA-10b Triggers the Epithelial–Mesenchymal Transition (EMT) of Laryngeal Carcinoma Hep-2 Cells by Directly Targeting the E-cadherin. Appl Biochem Biotechnol 176, 33–44 (2015). https://doi.org/10.1007/s12010-015-1505-6

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  • DOI: https://doi.org/10.1007/s12010-015-1505-6

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