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Production of Lactic Acid from the Mixture of Softwood Pre-hydrolysate and Paper Mill Sludge by Simultaneous Saccharification and Fermentation

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Abstract

Paper mill sludge is a solid waste material composed of pulp residues and ash generated from pulping and paper making process. The carbohydrate portion of the sludges from Kraft/Recycle paper mill has chemical and physical characteristics similar to those of commercial wood pulp. Because of its high carbohydrate content and well-dispersed structure, the sludge can be biologically converted to value-added products without pretreatment. In bioconversion of solid feedstock such as paper mill sludge, a certain amount of water must be present to attain fluidity. In this study, hemicellulose pre-hydrolysate, in place of water, was added to the sludge to increase the concentration of the final product. Pre-hydrolysate was obtained by hot-water treatment of pine wood in which the total sugar concentration reached 4 wt.%. The mixture was processed by simultaneous saccharification and fermentation (SSF) using enzymes (cellulase and pectinase) and Lactobacillus rhamnosus (ATCC-10863). Pectinase was added to hydrolyze mannose oligomers in the pre-hydrolysate to monomers. During the SSF of the mixture, calcium carbonate in the paper sludge acted as a buffer, yielding calcium lactate as the final product. External pH control was unnecessary due to the buffer action of calcium carbonate that maintained the pH near optimum for the SSF. The lactic acid yield in the range of 80–90 % of the theoretical maximum was obtained. Use of the mixed feed of pre-hydrolysate and pulp mill sludges in the SSF raised the product concentration to 60 g of lactate/L.

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Acknowledgments

We gratefully acknowledge the financial support provided by Alabama Center for Paper and Bioresources Engineering and Packaging Corporation of America, Chicago, IL. We also would like to thank Novozymes, North America for providing C-Tec2 enzyme, DuPont-Danisco for providing pectinase enzyme, and Boise Paper Company, Jackson, AL for providing the paper mill sludges.

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  1. Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL, 36849, USA

    Suan Shi, Li Kang & Y. Y. Lee

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  1. Suan Shi
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  2. Li Kang
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  3. Y. Y. Lee
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Correspondence to Y. Y. Lee.

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Shi, S., Kang, L. & Lee, Y.Y. Production of Lactic Acid from the Mixture of Softwood Pre-hydrolysate and Paper Mill Sludge by Simultaneous Saccharification and Fermentation. Appl Biochem Biotechnol 175, 2741–2754 (2015). https://doi.org/10.1007/s12010-014-1451-8

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  • DOI: https://doi.org/10.1007/s12010-014-1451-8

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