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Differential Display of Antioxidants in Mitigating Adverse Effects of UV-B Radiation in Nostoc muscorum and Phormidium foveolarum Photoacclimated to Different Irradiances

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Abstract

The response of UV-B radiation (UV-BL; 0.5) and (UV-BH; 1.5 μmol m−2 s−1) was investigated in two cyanobacteria—Nostoc muscorum and Phormidium foveolarum—preacclimated to low, normal, and high (LL; 15 ± 0.5, NL; 75 ± 3.5, and HL; 225 ± 5.5 μmol photon m−2 s−1, respectively) light intensities. Acclimation to HL stimulated growth over the control; however, both UV-B doses induced decline in growth of both cyanobacteria. UV-B-induced decline was maximum in LL acclimated cells which was in consonance with the results for chlorophyll content. LL acclimation led to an increase in phycocyanin content. Carotenoids content was maximum in HL acclimated (UV-B untreated and treated) cells of N. muscorum and P. foveolarum. Maximum stimulation in SOR and H2O2 levels was observed under LL + UV-BH treatment which was correlated with inefficient antioxidative mechanism (SOD, CAT, POD, and GST) in these cells. MDA and RCG contents also showed a similar trend. Mitigation of UV-B-induced stress response could be established in HL acclimated cells due to the concerted and differential display of enzymatic and nonenzymatic antioxidants in them. P. foveolarum proved to be more resistant than N. muscorum under the studied test conditions.

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Abbreviations

Car:

Carotenoids

Chl a :

Chlorophyll a

PAR:

Photosynthetically active radiation

PSI:

Photosystem I

PBS:

Phycobilisomes

Phy:

Phycocyanin

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Acknowledgments

The authors are thankful to The Head, Department of Botany, University of Allahabad, Allahabad for providing necessary laboratory facilities. The authors are also thankful to the University Grants Commission, New Delhi for providing financial support to carry out this work.

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  1. Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002, UP, India

    Gunjan Dubey & Sheo Mohan Prasad

  2. Department of Botany, University of Rajasthan, Jaipur, 302004, Rajasthan, India

    Gunjan Dubey

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Dubey, G., Prasad, S.M. Differential Display of Antioxidants in Mitigating Adverse Effects of UV-B Radiation in Nostoc muscorum and Phormidium foveolarum Photoacclimated to Different Irradiances. Appl Biochem Biotechnol 175, 2703–2728 (2015). https://doi.org/10.1007/s12010-014-1446-5

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