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Identification of Candida tropicalis BH-6 and Synergistic Effect with Pantoea agglomerans BH-18 on Hydrogen Production in Marine Culture

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Abstract

A marine yeast was isolated from mangrove sludge and named Candida tropicalis BH-6. The optimum temperature and the initial pH value for growth of the isolated strain were 37 °C and 5.0, respectively. The strain had high salt tolerance and could survive at NaCl concentrations of 0–6 %. Additionally, the yield of hydrogen production by C. tropicalis BH-6 was only 66.30 ml/l. However, when the yeast was mixed with Pantoea agglomerans BH-18, hydrogen production increased significantly to a maximum of 1707.5 ml/l, which was 36.94 and 247.54 % higher than the monoculture of P. agglomerans BH-18 and C. tropicalis BH-6, respectively. Taken together, these results revealed that in mixed culture, the yeast strain isolated from the same ecosystem as P. agglomerans BH-18 likely consumed the organic acids produced by fermentation, thus eliminating the factor inhibiting hydrogen production by P. agglomerans BH-18. As a result, the yield of hydrogen production during mixed culture increased significantly.

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Acknowledgments

The authors wish to acknowledge the financial support by the Tianjin Natural Science Foundation for Youths (No. 12JCQNJC04300 and No. 12JCQNJC04200), the Key Program of Tianjin Natural Science Foundation (No. 12JCZDJC22200), the Excellent Young Teachers Program of Tianjin (2014), the Doctoral Program Foundation of Higher education of China (20121208110001),  the National Natural Science Foundation of China (No. 40906074) 

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Authors and Affiliations

  1. College of Marine Science and Engineering, Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Daling Zhu, Guangce Wang & Guanghua Pan

  2. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao, 266071, People’s Republic of China

    Yingchao Ma & Guangce Wang

  3. College of Earth Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China

    Yingchao Ma

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  1. Daling Zhu
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  2. Yingchao Ma
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  3. Guangce Wang
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  4. Guanghua Pan
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Corresponding author

Correspondence to Guangce Wang.

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Daling Zhu and Yingchao Ma contributed equally to this study and share first authorship.

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Zhu, D., Ma, Y., Wang, G. et al. Identification of Candida tropicalis BH-6 and Synergistic Effect with Pantoea agglomerans BH-18 on Hydrogen Production in Marine Culture. Appl Biochem Biotechnol 175, 2677–2688 (2015). https://doi.org/10.1007/s12010-014-1436-7

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  • DOI: https://doi.org/10.1007/s12010-014-1436-7

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