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The Synthesis of a Novel Near-Infrared Fluorescent Probe and its Application in Imaging of Living Cells

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Abstract

IR-780, a novel near-infrared (NIR) fluorescent probe, was synthesized and applied to living cells. The probe exhibited good fluorescent characteristic, and cell experiments showed the probe had high affinity and without apparent cytotoxicity. Fluorescent image experiments in living A549 (Human lung adenocarcinoma epithelial cell line) and L929 (mouse fibroblast cell line) cells, further demonstrated its potential applications in biological systems. The probe effectively prevented the influence of autofluorescence and native cellular species in biological systems. It also exhibited excellent cell membrane permeability, good photostability, and high sensitivity.

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References

  1. Keramidas, M., Josserand, V., Righini, C. A., et al. (2010). British Journal of Surgery, 97, 737–743.

    Article  CAS  Google Scholar 

  2. Hilderbrand, S. A., & Weissleder, R. (2010). Current Opinion in Chemical Biology, 14, 71e9.

    Article  Google Scholar 

  3. Ogawa, M., Regino, C. A., Choyke, P. L., & Kobayashi, H. (2009). Molecular Cancer Therapeutics, 8, 232–239.

    Article  CAS  Google Scholar 

  4. Hsu, A. R., Hou, L. C., Veeravagu, A., et al. (2006). Molecular Imaging & Biology, 8, 315–323.

    Article  Google Scholar 

  5. Keramidas, R., Tung, C. H., Mahmood, U., & Bogdanov, A., Jr. (1999). Biotechnology, 17, 375–378.

    Google Scholar 

  6. Ogawa, M., Regino, C. A., Choyke, P. L., & Kobayashi, H. (2009). Molecular Cancer Therapeutics, 8, 232–239.

    Article  CAS  Google Scholar 

  7. Bjornsson, O. G., Murphy, R., & Chadwick, V. S. (1982). Experientia, 38, 1441–1442.

    Article  CAS  Google Scholar 

  8. Xiao, W., Yao, N., Peng, L., Liu, R., & Lam, K. S. (2009). European Journal of Nuclear Medicine and Molecular Imaging, 36, 94–103.

    Article  CAS  Google Scholar 

  9. Tang, B., Liu, X., Xu, K., Huang, H., Yang, G., & An, L. (2007). Chemical Communications, 36, 3726–3728.

    Article  Google Scholar 

  10. Huber, W., & Koella, J. C. (1993). Acta Tropica, 55, 257–261.

    Article  CAS  Google Scholar 

  11. Seidl, K., & Zinkernagel, A. S. (2013). Journal of Microbiological Methods, 92, 307–309.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work is supported by the National Basic Research Program of People’s Republic of China (No. 2011CB933503), Technology Supporting Program of Jiangsu province (BE2012657) and the Fundamental Research Funds for the Central Universities (2242014R30019).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 210096, China

    Chunlong Sun, Jin Cai, Junqing Chen, Yang Wu, Xi Zong & Yongfeng Lv

  2. School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China

    Peng Wang, Gaoxin Zhou, Bo Chen & Min Ji

Authors
  1. Chunlong Sun
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  2. Jin Cai
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  3. Junqing Chen
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  4. Yang Wu
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  5. Peng Wang
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  6. Gaoxin Zhou
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  7. Xi Zong
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  8. Bo Chen
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  9. Yongfeng Lv
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  10. Min Ji
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Correspondence to Min Ji.

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Sun, C., Cai, J., Chen, J. et al. The Synthesis of a Novel Near-Infrared Fluorescent Probe and its Application in Imaging of Living Cells. Appl Biochem Biotechnol 175, 1644–1650 (2015). https://doi.org/10.1007/s12010-014-1398-9

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  • DOI: https://doi.org/10.1007/s12010-014-1398-9

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