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Repetitive Gly-Leu-Lys-Gly-Glu-Asn-Arg-Gly-Asp Peptide Derived from Collagen and Fibronectin for Improving Cell–Scaffold Interaction

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Abstract

Suitable scaffolds for tissue engineering should provide a microenvironment for cell dwelling and directing cell behavior that resemble the native environment. Three-dimensional geometry of electrospun scaffolds well supports cell deposition, but they often lack biomacromolecules to induce cell responses. In this work, the repetitive collagen and fibronectin motif (rCF) peptide containing multiple repeats of Gly-Leu-Lys-Gly-Glu-Asn-Arg-Gly-Asp sequence derived from the cell adhesion motifs of collagen and fibronectin was produced as the alternative agent to induce cell–scaffold interaction. The DNA fragment encoding rCF peptide was amplified by a polymerase chain reaction using overlap primers without a DNA template, cloned into a protein expression vector, and expressed as a His–tag fusion peptide in Escherichia coli. The purified rCF peptide possessed cell adhesion activity about 1.5-fold of the commercial RGD peptide. The rCF peptide was grafted onto the electrospun PCL scaffold via RF plasma of Ar/O2 discharge and acrylic acid treatment. The immobilized rCF peptide significantly increased surface hydrophilicity and enhanced cell proliferation of the electrospun PCL scaffold. These findings suggest the potential application of rCF peptide for improving the biomimetic functions of polymeric scaffolds for tissue engineering.

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Abbreviations

rCF:

The repetitive collagen and fibronectin motif peptide

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Acknowledgments

This work was financially supported by the SUT Research and Development Support Fund. We also thank Assistant Professor B. Boonyapalanan for his kindness to allow us to use his electrospinning unit.

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Authors and Affiliations

  1. Health Science Program, Faculty of Science, Udon Thani Rajabhat University, Udon Thani, 41000, Thailand

    Patcharaporn Chaisri

  2. Department of Physics, Faculty of Science, Mahasarakham University, Mahasarakham, 44150, Thailand

    Artit Chingsungnoen

  3. School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand

    Sineenat Siri

Authors
  1. Patcharaporn Chaisri
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  2. Artit Chingsungnoen
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  3. Sineenat Siri
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Correspondence to Sineenat Siri.

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Chaisri, P., Chingsungnoen, A. & Siri, S. Repetitive Gly-Leu-Lys-Gly-Glu-Asn-Arg-Gly-Asp Peptide Derived from Collagen and Fibronectin for Improving Cell–Scaffold Interaction. Appl Biochem Biotechnol 175, 2489–2500 (2015). https://doi.org/10.1007/s12010-014-1388-y

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  • DOI: https://doi.org/10.1007/s12010-014-1388-y

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