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A New Amperometric Biosensor Based on Fe3O4/Polyaniline/Laccase/Chitosan Biocomposite-Modified Carbon Paste Electrode for Determination of Catechol in Tea Leaves

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Abstract

In the present study, a new biosensor based on laccase from Paraconiothyrium variabile was developed for catechol. The purified enzyme entrapped into the Fe3O4/polyaniline/chitosan (Fe3O4/polyaniline (PANI)/chitosan (CS)) biocomposite matrix film without the aid of other cross-linking reagents by a one-step electrodeposition on the surface of carbon paste electrode (CPE). The formed layer of biocomposite was characterized with scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV). The biosensor was optimized with respect to biocomposite composition, enzyme loading, and solution pH by amperometry method. The biosensor exhibited noticeable eletrocatalytic ability toward catechol with a linear concentration range from 0.5 to 80 μM and a detection limit of 0.4 μM. The biosensor showed optimum response within 8 s, at pH 5, and 40 °C. The apparent Michaelis–Menten (K M app) was found to be 1.092 μM. The fabricated biosensor could be applied for determination of catechol in tea leaf samples.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, University of Birjand, Birjand, South Khorasan, Iran

    Susan Sadeghi & Ebrahim Fooladi

  2. Department of Clinical Biochemistry, Birjand University of Medical Sciences, Birjand, South Khorasan, Iran

    Mohammad Malekaneh

Authors
  1. Susan Sadeghi
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  2. Ebrahim Fooladi
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  3. Mohammad Malekaneh
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Correspondence to Susan Sadeghi.

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Sadeghi, S., Fooladi, E. & Malekaneh, M. A New Amperometric Biosensor Based on Fe3O4/Polyaniline/Laccase/Chitosan Biocomposite-Modified Carbon Paste Electrode for Determination of Catechol in Tea Leaves. Appl Biochem Biotechnol 175, 1603–1616 (2015). https://doi.org/10.1007/s12010-014-1380-6

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  • DOI: https://doi.org/10.1007/s12010-014-1380-6

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