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Improving Enzymatic Hydrolysis of Corn Stover Pretreated by Ethylene Glycol-Perchloric Acid-Water Mixture

Applied Biochemistry and Biotechnology volume 175, pages 1306–1317 (2015)Cite this article

Abstract

To improve the enzymatic saccharification of lignocellulosic biomass, a mixture of ethylene glycol-HClO4-water (88.8:1.2:10, w/w/w) was used for pretreating corn stover in this study. After the optimization in oil-bath system, the optimum pretreatment temperature and time were 130 °C and 30 min, respectively. After the saccharification of 10 g/L pretreated corn stover for 48 h, the saccharification rate was obtained in the yield of 77.4 %. To decrease pretreatment temperature and shorten pretreatment time, ethylene glycol-HClO4-water (88.8:1.2:10, w/w/w) media under microwave irradiation was employed to pretreat corn stover effectively at 100 °C and 200 W for 5 min. Finally, the recovered hydrolyzates containing glucose obtained from the enzymatic hydrolysis of pretreated corn stovers could be fermented into ethanol efficiently. These results would be helpful for developing a cost-effective pretreatment combined with enzymatic saccharification of cellulosic materials for the production of lignocellulosic ethanol.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 21102011), the Natural Science Foundation of Jiangsu Province (No. BK20141172) and the Open Project Program of the State Key Laboratory of Bioreactor Engineering, and the Key Laboratory of Guangxi Biorefinery (No. GXBF11-03).

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Affiliations

  1. Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, 213164, China

    Yu-Cai He, Feng Liu, Lei Gong, Ting Lu, Yun Ding, Dan-Ping Zhang, Qing Qing & Yue Zhang

  2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Yu-Cai He

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  1. Yu-Cai He
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  2. Feng Liu
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Correspondence to Yu-Cai He.

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He, YC., Liu, F., Gong, L. et al. Improving Enzymatic Hydrolysis of Corn Stover Pretreated by Ethylene Glycol-Perchloric Acid-Water Mixture. Appl Biochem Biotechnol 175, 1306–1317 (2015). https://doi.org/10.1007/s12010-014-1353-9

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  • DOI: https://doi.org/10.1007/s12010-014-1353-9

Keyword

  • Corn stover
  • Ethylene glycol
  • Perchloric acid
  • Pretreatment
  • Saccharification