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Silica Nanoparticles Induced Metabolic Stress through  EGR1, CCND, and E2F1 Genes in Human Mesenchymal Stem Cells

Applied Biochemistry and Biotechnology volume 175, pages 1181–1192 (2015)Cite this article

Abstract

The SiO2 synthesized in bulk form, adopting the conventional methods for application in food industry applications, may also contain nano-sized particles. On account of the unique physico-chemical properties, the SiO2 particulates, such as size and shape, cause metabolic toxicity in cells. Poor understanding of the molecular level nanotoxicity resulting from high-volume synthetic SiO2 exposures in humans is a serious issue, since these particles may also contribute to metabolic stress-mediated chronic diseases. In the present study, we examined the structural characteristics of these nano-sized silica particles adopting SEM and dynamic light scattering (DLS) and assessed the alterations in the cell cycle induced by these silica particles in human mesenchymal stem cells (hMSCs) adopting 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay, morphological changes in the cells adopting fluorescent microscopy, cell cycle analysis adopting flow cytometry, and the expression of genes linked to cell cycle (i.e., proliferating cell nuclear antigen (PCNA), early growth response protein (EGR1), E2F transcription factor (E2F1), cyclin D1, cyclin C, and cyclin D3) adopting qPCR. The SEM and DLS studies indicated that the commercial grade SiO2-NPs were in the nano-scale range. Alterations in the cytoplasmic organization, nuclear morphology, cell cycle progression, and expression of genes linked to cell cycle-dependent metabolic stress through EGR1, CCND, and E2F1 genes were the primary indicators of metabolic stress. Overall, the results of this study demonstrate that synthetic SiO2 acutely affects hMSC through cell cycle-dependent oxidative stress gene network. The toxicity mechanisms (both acute and chronic) of food grade silica should be investigated in greater depth with special reference to food safety.

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Acknowledgments

We gratefully acknowledge the financial support from the National Program for Science and Technology, King Saud University, Saudi Arabia (Project code: BIO-981-10).

Conflict of Interest

None declared.

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Authors and Affiliations

  1. Nanobiotechnology and Molecular Biology Research Lab, Department of Food Science and Nutrition, College of Food Science and Agriculture, Riyadh, Saudi Arabia

    Vaiyapuri S. Periasamy, Jegan Athinarayanan, Mohammad A. Akbarsha & Ali A. Alshatwi

  2. Mahatma Gandhi-Doerenkamp Center, Bharathidasan University, Tiruchirappalli, 620024, India

    Mohammad A. Akbarsha

  3. Department of Food Science and Nutrition, College of Food Sciences and Agriculture, King Saud University, P.O. Box 2460, Riyadh, 11451, Kingdom of Saudi Arabia

    Ali A. Alshatwi

Authors
  1. Vaiyapuri S. Periasamy
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  2. Jegan Athinarayanan
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  3. Mohammad A. Akbarsha
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  4. Ali A. Alshatwi
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Correspondence to Ali A. Alshatwi.

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Periasamy, V.S., Athinarayanan, J., Akbarsha, M.A. et al. Silica Nanoparticles Induced Metabolic Stress through  EGR1, CCND, and E2F1 Genes in Human Mesenchymal Stem Cells. Appl Biochem Biotechnol 175, 1181–1192 (2015). https://doi.org/10.1007/s12010-014-1342-z

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  • DOI: https://doi.org/10.1007/s12010-014-1342-z

Keywords

  • SiO2 nano-particles
  • Oxidative stress
  • Cell cycle
  • Mesenchymal stem cells