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The Effect of Heavy Metal-Induced Oxidative Stress on the Enzymes in White Rot Fungus Phanerochaete chrysosporium

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Abstract

Prevalence of heavy metals in the living environment causes chemical stress and reactive oxygen species (ROS) formation in Phanerochaete chrysosporium (P. chrysosporium). However, the mechanisms involved in ROS defense are still under investigation. In the present study, we evaluated the effect of lead- and cadmium-induced oxidative stress on the activities of catalase (CAT), peroxidase (POD), lignin peroxidase (LiP), and manganese peroxidase (MnP). A time-dependent change in all enzyme activities was observed following exposure to 50 μM cadmium and 25 μM lead. The lowest values were recorded at 4 h after exposure. Both cadmium and lead inhibited CAT and POD. The cytochrome P450 (CYP450) levels increased under 50–100 μM cadmium or lead exposure and decreased when heavy metal concentration was under 50 μM; this suggested that ROS is not the only factor that alters the CYP450 levels. The cadmium removal rate in the sample containing 900 μM taxifolin (inhibitor of CYP450) and 100 μM cadmium was reduced to 12.34 %, 9.73 % lower than that of 100 μM cadmium-induced sample, indicating CYP450 may play an indirect but key role in the process of clearance of heavy metals. The pH of the substrate solution decreased steadily during the incubation process.

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Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (51039001, 51178171), the Program for New Century Excellent Talents in University (NCET-10-0361), and the Research Fund for the Doctoral Program of Higher Education of China (20100161110012).

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People’s Republic of China

    Qihua Zhang, Guangming Zeng, Guiqiu Chen, Min Yan, Anwei Chen, Jianjian Du, Jian Huang, Bin Yi, Ying Zhou, Xiaoxiao He & Yan He

  2. Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha, 410082, People’s Republic of China

    Qihua Zhang, Guangming Zeng, Guiqiu Chen, Min Yan, Anwei Chen, Jianjian Du, Jian Huang, Bin Yi, Ying Zhou, Xiaoxiao He & Yan He

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  1. Qihua Zhang
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  2. Guangming Zeng
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  4. Min Yan
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Correspondence to Guangming Zeng or Guiqiu Chen.

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Zhang, Q., Zeng, G., Chen, G. et al. The Effect of Heavy Metal-Induced Oxidative Stress on the Enzymes in White Rot Fungus Phanerochaete chrysosporium . Appl Biochem Biotechnol 175, 1281–1293 (2015). https://doi.org/10.1007/s12010-014-1298-z

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  • DOI: https://doi.org/10.1007/s12010-014-1298-z

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