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Formulation and Characterisation of Antibody-Conjugated Soy Protein Nanoparticles—Implications for Neutralisation of Snake Venom with Improved Efficiency

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Abstract

The present study reports the formulation of soy protein nanoparticles and its conjugation to antivenom. The conditions for nanoparticle formation were optimised by considering particle size, count rate, stability and zeta potential. The smallest particle size of 70.9 ± 0.9 nm with a zeta potential of −28.0 ± 1.4 mV was obtained at pH 6.2, with NaOH 5.4 % and 28 μg/mg glutaraldehyde. The nanoparticle was conjugated with antisnake venom immunoglobulins (F(ab′)2 fragments) using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide. TEM analysis indicated the increased size of particle to 600 nm after conjugation to antivenom. Further, in vitro studies indicated that conjugated antibodies inhibited the activity of protease, phospholipase and hyaluronidase enzymes of Bungarus caeruleus venom more efficiently than the free antivenom. This is the first report on the use of protein nanoparticles for conjugating snake venom antibodies and their implications for neutralising snake venom enzymes with increased efficiency.

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Acknowledgments

The authors are thankful to the management of VIT University for providing the infrastructure and support. The authors are also thankful to IISc, Bangalore and IIT Bombay, Mumbai, for Particle size analyser and TEM analysis. The generous gift of soya flake by Saptagiri Farm Food, Port Blair is also acknowledged.

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Authors and Affiliations

  1. School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India

    Kadali Renu, Kadiyala Gopi & Gurunathan Jayaraman

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  1. Kadali Renu
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  2. Kadiyala Gopi
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  3. Gurunathan Jayaraman
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Corresponding author

Correspondence to Gurunathan Jayaraman.

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Renu, K., Gopi, K. & Jayaraman, G. Formulation and Characterisation of Antibody-Conjugated Soy Protein Nanoparticles—Implications for Neutralisation of Snake Venom with Improved Efficiency. Appl Biochem Biotechnol 174, 2557–2570 (2014). https://doi.org/10.1007/s12010-014-1207-5

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