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RETRACTED ARTICLE: Genome Shuffling Enhances Lipase Production of Thermophilic Geobacillus sp.

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This article was retracted on 03 June 2017

Abstract

Thermostable lipases are potential enzymes for biocatalytic application. In this study, the lipase production of Geobacillus sp. CF03 (WT) was improved by genome shuffling. After two rounds of genome shuffling, one fusant strain (FB1) achieved increase lipase activity from the populations generated by ultraviolet irradiation and ethyl methylsulfonate (EMS) mutagenesis. The growth rate and lipase production of FB1 increased highest by 150 and 238 %, respectively, in comparison to the wild type. The fusant enzyme had a significant change in substrate specificity but still prefers the long-chain length substrates. It had an optimum activity at 60 °C, pH at 7.0–8.0, with p-nitrophenyl palmitate (C16) as a substrate and retained about 50 % of their activity after 15 min at 70 °C, pH 8.0. Furthermore, the fusant lipase showed the preference of sesame oil, waste palm oil, and canola oil. Therefore, the genome shuffling strategy has been successful to strain improvement and selecting strain with multiple desirable characteristics.

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References

  1. McMullan, G., Christie, J. M., Rahman, T. J., Banat, I. M., Ternan, N. G., & Marchant, R. (2004). Biochemical Society Transactions, 32, 214–217.

    Article  CAS  Google Scholar 

  2. Dheeran, P., Kumar, S., Jaiswal, Y. K., & Adhikari, D. K. (2010). Applied Microbiology and Biotechnology, 86, 1857–1866.

    Article  CAS  Google Scholar 

  3. Sookkheo, B., Sinchaikul, S., Phutrakul, S., & Chen, S. T. (2000). Protein Expression and Purification, 20, 142–151.

    Article  CAS  Google Scholar 

  4. Lee, D. W., Kim, H. W., Lee, K. W., Kim, B. C., Choe, E. A., Lee, H. S., Kim, D. S., & Pyun, Y. R. (2001). Enzyme and Microbial Technology, 29, 363–371.

    Article  CAS  Google Scholar 

  5. Sharma, R., Chisti, Y., & Banerjee, U. C. (2001). Biotechnology Advances, 19, 627–662.

    Article  CAS  Google Scholar 

  6. Singh, A. K., & Mukhopadhyay, M. (2012). Applied Biochemistry and Biotechnology, 166, 486–520.

    Article  CAS  Google Scholar 

  7. Zhang, Y. X., Perry, K., Vinci, V. A., Powell, K., Stemmer, W. P. C., & del Cardayré, S. B. (2002). Nature, 415, 644–646.

    Article  CAS  Google Scholar 

  8. Gong, J., Zheng, H., Wu, Z., Chen, T., & Zhao, X. (2009). Biotechnology Advances, 27, 996–1005.

    Article  Google Scholar 

  9. Zhang, Y., Liu, J. Z., Huang, J. S., & Mao, Z. W. (2010). Journal of Biotechnology, 148, 139–143.

    Article  CAS  Google Scholar 

  10. Jian, X. K., Chen, X. J., Chen, W. R., Li, M. S., Fang, Y., & Li, D. S. (2011). Process Biochemistry, 46, 792–795.

    Article  Google Scholar 

  11. Patnaik, R., Louie, S., Gavrilovic, V., Perry, K., Stemmer, W. P., & Ryan, C. M. (2002). Nature Biotechnology, 20, 707–712.

    Article  CAS  Google Scholar 

  12. Wang, Y. H., Li, Y., Pei, X. L., Yu, L., & Feng, Y. (2007). Journal of Biotechnology, 129, 510–515.

    Article  CAS  Google Scholar 

  13. Samad, M. Y. A., Razak, C. N. A., Salleh, A. B., Zin Wan Yunus, W. M., Ampon, K., & Basri, M. (1989). Journal of Microbiological Methods, 8, 51–56.

    Article  Google Scholar 

  14. Lane, D. J. (1991). In nucleic acid techniques in bacteria systematics. NY: Wiley.

    Google Scholar 

  15. Shu, C. H., Xu, C. J., & Lin, G. C. (2006). Process Biochemistry, 41, 734–738.

    Article  CAS  Google Scholar 

  16. Aly, M. M., Tork, S., Al-Garni, S. M., & Nawar, L. (2012). African Journal of Microbiology Research, 6, 1125–1137.

    Article  CAS  Google Scholar 

  17. Kang, J. X., Chen, X. J., Chen, W. R., Li, M. S., Fang, Y., Li, D. S., Ren, Y. Z., & Liu, D. Q. (2011). Process Biochemistry, 46, 792–795.

    Article  CAS  Google Scholar 

  18. Yu, L., Pei, X., Lei, T., Wang, Y., & Feng, Y. (2008). Journal of Biotechnology, 134, 154–159.

    Article  CAS  Google Scholar 

  19. Simons, J. W., Van Kampen, M. D., Ubarretxena-Belandia, I., Cox, R. C., Alves dos Santos, C. M., Egmond, M. R., & Verheij, H. M. (1999). Biochemistry, 38, 2–10.

    Article  CAS  Google Scholar 

  20. Tyndall, J. D., Sinchaikul, S., Fothergill-Gilmore, L. A., Taylor, P., & Walkinshaw, M. D. (2002). Journal of Molecular Biology, 323, 859–869.

    Article  CAS  Google Scholar 

  21. Salameh, M. A., & Wiegel, J. (2007). Applied and Environmental Microbiology, 73, 7725–7731.

    Article  CAS  Google Scholar 

  22. Uttatree, S., Winayanuwattikun, P., & Charoenpanich, J. (2010). Applied Biochemistry and Biotechnology, 162, 1362–1376.

    Article  CAS  Google Scholar 

  23. Cho, A. R., Yoo, S. K., & Kim, E. J. (2000). FEMS Microbiology Letters, 186, 235–238.

    Article  CAS  Google Scholar 

  24. Schmidt-Dannert, C., Rua, M. L., Atomi, H., & Schmid, R. D. (1996). Biochimica et Biophysica Acta, 1301, 105–114.

    Article  Google Scholar 

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Acknowledgments

This research was supported financially by National Research Council of Thailand (NRCT), Thailand for PC, The Centre of Excellence on Environmental Health and Toxicology, Science & Technology Postgraduate Education and Research Development Office (PERDO), Ministry of Education (Grant no. ETM-R-05-003/2554) for JC, and Kasetsart University and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission for KC.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan Rd, Chatuchak, Bangkok, 10900, Thailand

    Pornchanok Chalopagorn & Kiattawee Choowongkomon

  2. Department of Biochemistry and Centre of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Burapha University, Bangsaen, Chonburi, 20131, Thailand

    Jittima Charoenpanich

  3. Environmental Science Program, Faculty of Science, Burapha University, Bangsaen, Chonburi, 20131, Thailand

    Jittima Charoenpanich

  4. Center of Excellence on Environmental Health and Toxicology (CHE), Ministry of Education, Bangkok, Thailand

    Jittima Charoenpanich

  5. Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Thailand CASTNAR, NRU-KU, Bangkok, Thailand

    Kiattawee Choowongkomon

Authors
  1. Pornchanok Chalopagorn
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  2. Jittima Charoenpanich
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  3. Kiattawee Choowongkomon
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Corresponding authors

Correspondence to Jittima Charoenpanich or Kiattawee Choowongkomon.

Additional information

Per the request of the Faculty of Science, Kasetsart University, who conducted an internal investigation, this article has been retracted due a conflict concerning the origin of science discussed in this article

An erratum to this article is available at http://dx.doi.org/10.1007/s12010-017-2516-2.

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Chalopagorn, P., Charoenpanich, J. & Choowongkomon, K. RETRACTED ARTICLE: Genome Shuffling Enhances Lipase Production of Thermophilic Geobacillus sp.. Appl Biochem Biotechnol 174, 1444–1454 (2014). https://doi.org/10.1007/s12010-014-1109-6

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  • DOI: https://doi.org/10.1007/s12010-014-1109-6

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