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Oxygen Transfer in Solid-State Cultivation Under Controlled Moisture Conditions

Applied Biochemistry and Biotechnology volume 174, pages 708–718 (2014)Cite this article

Abstract

The aim of this work was to study oxygen transfer as a function of the initial moisture content in solid-state cultivation under controlled moisture conditions. The use of controlled moisture conditions prevents drastic changes in the medium during cultivation, allowing the use of a pseudo-steady-state model to estimate the overall oxygen mass transfer coefficient (K L a) in the biofilm around the solid particles. Drechslera (Helminthosporium) monoceras, an aerobic mold that produces allergenic proteins, was cultured on wheat bran in a packed bed column bioreactor. The bed height (30 mm) and air flow rate (0.4 L/min) were selected to implement moisture control. The results show that there is an optimal moisture content (35 %) at which a lower biofilm thickness and packing of the bed improves K L a. However, a higher biomass growth was obtained at 45 % moisture. The different patterns of biomass growth demonstrate the importance of the balance between aerial and film growth in solid-state cultivation. These results contribute to the understanding of oxygen transfer in solid fermentation, optimization of processes, and production of allergen extracts from D. (Helminthosporium) monoceras biomass.

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Abbreviations

φ :

Bed porosity (%)

ρ bulk :

Bulk density (g/cm3)

ρ real :

Solid real density (g/cm3)

C * :

Equilibrium oxygen concentration [mol/m3]

C G :

Oxygen concentration in gas phase [mol/m3]

C L :

Oxygen concentration in liquid film [mg/L]

\( {D}_{{\mathrm{O}}_2,\ \mathrm{L}} \) :

Oxygen diffusivity in liquid phase [m2/s]

F :

Air flow rate [L/min]

K L a :

Overall oxygen transfer coefficient [s−1]

N :

Overall oxygen transfer rate [mol/s m3]

R 1 :

Radius of solid particle alone [mm]

R 2 :

Radius of solid particle with the surrounding liquid film [mm]

\( {R}_{{\mathrm{O}}_2} \) :

Oxygen consumption rate per biofilm volume [mol/s m3]

V S :

Volume of solid medium [m3]

References

  1. Ooijkaas, L. P., Weber, F. J., Buitelaar, R. M., Tramper, J., & Rinzema, A. (2002). Trends in Biotechnology, 18, 355–360.

    Google Scholar 

  2. Pandey, A. (2003). Biochemical Engineering Journal, 13, 81–84.

    Article  CAS  Google Scholar 

  3. Singhania, R. R., Patel, A. K., Soccol, C. R., & Pandey, A. (2009). Biochemical Engineering Journal, 44, 13–18.

    Article  CAS  Google Scholar 

  4. Raghavarao, K. S. M. S., Ranganathan, T. V., & Karanth, N. G. (2003). Biochemical Engineering Journal, 13, 127–135.

    Article  CAS  Google Scholar 

  5. Rokem, S. (1984). In H. W. Doelle, S. Rokem, & M. Berovic (Eds.), Biotechnology, vol. 6: industrial mycology (pp. 75–97). Oxford: Eolss.

    Google Scholar 

  6. Lekanda, J. S., & Pérez-Correa, J. R. (2004). Process Biochemistry, 39, 1793–1802.

    Article  CAS  Google Scholar 

  7. Gowthaman, M. K., Raghava Rao, K. S., & Karanth, N. G. (1993). Biotechnology Advances, 11, 611–620.

    Article  CAS  Google Scholar 

  8. Oostra, J., Le Comte, E. P., Van Der Heuvel, J. C., Tramper, J., & Rinzema, A. (2001). Biotechnology and Bioengineering, 75, 13–24.

    Article  CAS  Google Scholar 

  9. Roopesh, K., Ramachandran, S., Nampoothiri, K. M., Szakacs, G., & Pandey, A. (2006). Bioresource Technology, 97, 506–511.

    Article  CAS  Google Scholar 

  10. Delabona, P. D. S., Pirota, R. D. P. B., Codima, C. A., Tremacoldi, C. R., Rodrigues, A., & Farinas, C. S. (2013). Industrial Crops and Products, 42, 236–242.

    Article  CAS  Google Scholar 

  11. Rodríguez-Fernández, D. E., Rodríguez-León, J. A., De Carvalho, J. C., Karp, S. G., Sturm, W., Parada, J. L., & Soccol, C. R. (2012). Bioresource Technology, 118, 603–606.

    Article  Google Scholar 

  12. Gowthaman, M. K., Raghava Rao, K. S. M. S., Ghidyal, N. P., & Karanth, N. G. (1995). Process Biochemistry, 30, 9–15.

    Article  CAS  Google Scholar 

  13. Thibault, J., Pouliot, K., Agosin, E., & Pérez-Correa, R. (2000). Process Biochemistry, 36, 9–18.

    Article  CAS  Google Scholar 

  14. Rahardjo, Y. S. P., Weber, F. J., Paul Le Comte, E., Tramper, J., & Rinzema, A. (2002). Biotechnology and Bioengineering, 78, 539–544.

    Article  Google Scholar 

  15. Hasan, S. D. M., Zolner, R., & Santana, M. H. A. (2003). Applied Biochemistry and Biotechnology, 106, 403–412.

    Article  Google Scholar 

  16. Menezes, E. A., Gambale, W., Macedo, M., Abdalla, D., Paula, C. R., & Croce, J. (1995). Mycopathology, 131, 75–81.

    Article  CAS  Google Scholar 

  17. Bradford, M. M. (1976). Analytical Biochemistry, 72, 248–254.

    Article  CAS  Google Scholar 

  18. Perez-Garcia, O., de-Bashan, L. E., Hernandez, J. P., & Bashan, Y. (2010). Journal of Phycology, 46, 800–812.

    Article  CAS  Google Scholar 

  19. Shojaosadati, S. A., & Babaeipour, V. (2002). Process Biochemistry, 37, 909–914.

    Article  CAS  Google Scholar 

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Acknowledgments

The authors gratefully acknowledge Prof. Walderez Gamballe, who generously provided the fungus strain, and the Brazilian agencies CNPq and CAPES in addition to the State University of Campinas (UNICAMP).

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Authors and Affiliations

  1. Development of Biotechnological Processes Laboratory, School of Chemical Engineering, University of Campinas, Campinas, São Paulo, 13083-852, Brazil

    R. G. Bastos, F. L. Motta & M. H. A. Santana

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  1. R. G. Bastos
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  2. F. L. Motta
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  3. M. H. A. Santana
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Correspondence to M. H. A. Santana.

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Bastos, R.G., Motta, F.L. & Santana, M.H.A. Oxygen Transfer in Solid-State Cultivation Under Controlled Moisture Conditions. Appl Biochem Biotechnol 174, 708–718 (2014). https://doi.org/10.1007/s12010-014-1101-1

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Keywords

  • Solid-state cultivation
  • Drechslera (Helminthosporium) monoceras
  • Oxygen transfer
  • K L a
  • Packed bed bioreactor
  • Wheat bran