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Identification and Characterization of the Yellow Pigment Synthesized by Cupriavidus sp. USMAHM13

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Abstract

Microbial pigments are gaining intensive attention due to increasing awareness of the toxicity of synthetic colours. In this study, a novel polymer-producing bacterium designated as Cupriavidus sp. USMAHM13 was also found to produce yellow pigment when cultivated in nutrient broth. Various parameters such as temperature, pH and ratio of culture volume to flask volume were found to influence the yellow pigment production. UV-Visible, Fourier transform infrared and 13C-nuclear magnetic resonance analyses revealed that the crude yellow pigment might probably represent new bioactive compound in the carotenoid family. The crude yellow pigment also exhibited a wide spectrum of antimicrobial activity against Gram-negative and Gram-positive bacteria with their inhibition zones and minimal inhibitory concentrations ranged from 25 to 38 mm and from 0.63 to 2.5 mg/ml, respectively. To the best of our knowledge, this is the first report on the identification and characterization of yellow pigment produced by bacterium belonging to the genus Cupriavidus.

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Acknowledgements

This work was supported by the FRGS grant from Ministry of Education, Malaysia (203/PBIOLOGI/6711357) and USM Science Fellowship (RU: 1001/441/29301/CIPS/AUPE001) awarded to Hema Ramachandran.

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Authors and Affiliations

  1. School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    Hema Ramachandran & A.-A. Amirul

  2. Quest International University Perak, 30250, Ipoh, Perak, Malaysia

    Hema Ramachandran

  3. School of Chemical Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    Mohd Adnan Iqbal

  4. Centre for Chemical Biology, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    A.-A. Amirul

  5. Malaysian Institute of Pharmaceuticals and Nutraceuticals, MOSTI, 11700, Gelugor, Penang, Malaysia

    A.-A. Amirul

Authors
  1. Hema Ramachandran
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  2. Mohd Adnan Iqbal
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  3. A.-A. Amirul
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Correspondence to A.-A. Amirul.

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Ramachandran, H., Iqbal, M.A. & Amirul, AA. Identification and Characterization of the Yellow Pigment Synthesized by Cupriavidus sp. USMAHM13. Appl Biochem Biotechnol 174, 461–470 (2014). https://doi.org/10.1007/s12010-014-1080-2

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  • DOI: https://doi.org/10.1007/s12010-014-1080-2

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