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Effect of Acetic Acid on Citric Acid Fermentation in an Integrated Citric Acid–Methane Fermentation Process

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Abstract

An integrated citric acid–methane fermentation process was proposed to solve the problem of extraction wastewater in citric acid fermentation process. Extraction wastewater was treated by anaerobic digestion and then recycled for the next batch of citric acid fermentation to eliminate wastewater discharge and reduce water resource consumption. Acetic acid as an intermediate product of methane fermentation was present in anaerobic digestion effluent. In this study, the effect of acetic acid on citric acid fermentation was investigated and results showed that lower concentration of acetic acid could promote Aspergillus niger growth and citric acid production. 5-Cyano-2,3-ditolyl tetrazolium chloride (CTC) staining was used to quantify the activity of A. niger cells, and the results suggested that when acetic acid concentration was above 8 mM at initial pH 4.5, the morphology of A. niger became uneven and the part of the cells’ activity was significantly reduced, thereby resulting in deceasing of citric acid production. Effects of acetic acid on citric acid fermentation, as influenced by initial pH and cell number in inocula, were also examined. The result indicated that inhibition by acetic acid increased as initial pH declined and was rarely influenced by cell number in inocula.

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References

  1. Chang, V. S., & Holtzapple, M. T. (2000). Applied Biochemistry and Biotechnology, 84–86, 5–37.

    Article  Google Scholar 

  2. Zhi, X., Yang, H., Berthold, S., Doetsch, C., & Shen, J. (2010). Journal of Power Sources, 195, 6945–6953.

    Article  CAS  Google Scholar 

  3. Li, C., Yang, H. L., Xia, X., Li, Y., Chen, L., Zhang, M., Zhang, L., & Wang, W. (2013). Bioresource Technology, 127, 248–255.

    Article  CAS  Google Scholar 

  4. Zhu, Y. R., Zhou, C., Wang, J. H., Xie, H. S., & Gao, M. X. (2004). Techniques and Equipment for Environmental Pollution Control (In Chinese), 5, 64–66.

  5. Luo, S. R. (1996). Environmental Engineering (Chinese), 14, 8–10.

    CAS  Google Scholar 

  6. Wang, X. H., Guan, X. J., Xu, S. J., & Zhong, Y. Q. (2006). Technological Water Treatment (Chinese), 32, 61–65.

    CAS  Google Scholar 

  7. Zhu, L. H., Xu, X., & Wang, R. (2007). Industrial Water Treatment (Chinese), 27, 39–41.

    CAS  Google Scholar 

  8. Kayombo, S., Mbwette, T. S. A., Katima, J. H. Y., & Jorgensen, S. E. (2003). Water Research, 37, 2937–2943.

    Article  CAS  Google Scholar 

  9. Cheng, T., & Lin, T. (2008). GuiZhou Chemical Industry (Chinese), 33, 41–42.

    Google Scholar 

  10. Fu, G. L., Xu, X. Y., Fan, L. H., & Zhang, M. (2007). Journal of Huaihai Institute of Technology (Chinese), 16, 44–46.

    CAS  Google Scholar 

  11. Shi, Z. L., Li, W., & Yao, S. H. (2009). Journal of Shenyang University Chemical Technology (Chinese), 23, 289–293.

    CAS  Google Scholar 

  12. Qi, H., Cheng, F., Zhang, H., & Yang, J. (2001). Proceedings of the Third Asia-Pacific Conference on Sustainable Energy and Environmental Technologies. Hong Kong: World Scientific Publishing Company.

    Google Scholar 

  13. Tian, W. J., & Jiang, J. (2001). Journal of Shandong Institute of Mining and Technology (Chinese), 20, 117–120.

    CAS  Google Scholar 

  14. Alkaya, E., Kaptan, S., Ozkan, L., Uludag-Demirer, S., & Demirer, G. N. (2009). Chemosphere, 77, 1137–1142.

    Article  CAS  Google Scholar 

  15. Pavlostathis, S., & Giraldo-Gomez, E. (1991). Critical Reviews in Environmental Science and Technology, 21, 411–490.

    CAS  Google Scholar 

  16. Xu, J., Chen, Y. Q., Zhang, H. J., Tang, L., Wang, K., Zhang, J. H., Chen, X. S., & Mao, Z. G. (2014). Bioprocess and Biosystems Engineering. doi:10.1007/s00449-014-1138-0.

    Google Scholar 

  17. Thomas, K., Hynes, S., & Ingledew, W. (2002). Applied and Environmental Microbiology, 68, 1616–1623.

    Article  CAS  Google Scholar 

  18. Cássio, F., Leao, C., & Van Uden, N. (1987). Applied and Environmental Microbiology, 53, 509–513.

    Google Scholar 

  19. Casal, M., Cardoso, H., & Leao, C. (1996). Microbiology, 142, 1385–1390.

    Article  CAS  Google Scholar 

  20. Verduyn, C., Postma, E., Scheffers, W. A., & Vandijken, J. P. (1990). Journal of General Microbiology, 136, 405–412.

    Article  CAS  Google Scholar 

  21. Abbott, D. A., & Ingledew, W. (2004). Biotechnology Letters, 26, 1313–1316.

    Article  CAS  Google Scholar 

  22. Taherzadeh, M. J., Niklasson, C., & Lidén, G. (1997). Chemical Engineering Science, 52, 2653–2659.

    Article  CAS  Google Scholar 

  23. Kornberg, H., & Gotto, A. (1961). Biochemical Journal, 78, 69.

    CAS  Google Scholar 

  24. Jernejc, K., & Legiša, M. (2004). Journal of Biotechnology, 112, 289–297.

    Article  CAS  Google Scholar 

  25. Papagianni, M. (2007). Biotechnology Advances, 25, 244–263.

    Article  CAS  Google Scholar 

  26. Papagianni, M., & Mattey, M. (2006). Microbial Cell Factories, 5, 3.

    Article  Google Scholar 

  27. Papagianni, M., Mattey, M., & Kristiansen, B. (1999). Process Biochemistry, 35, 359–366.

    Article  CAS  Google Scholar 

  28. Pampulha, M., & Loureiro-Dias, M. (1990). Applied Microbiology and Biotechnology, 34, 375–380.

    Article  CAS  Google Scholar 

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Acknowledgments

This research was supported by Henan Tianguan Co., Ltd., China, and Yixing Xielian Biological Chemical Co., Ltd., China. We are thankful for their supports.

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China

    Jian Xu, Yang-Qiu Chen, Hong-Jian Zhang, Lei Tang, Ke Wang, Jian-Hua Zhang, Xu-Sheng Chen & Zhong-Gui Mao

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  1. Jian Xu
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  2. Yang-Qiu Chen
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  3. Hong-Jian Zhang
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  4. Lei Tang
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  5. Ke Wang
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Correspondence to Zhong-Gui Mao.

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Xu, J., Chen, YQ., Zhang, HJ. et al. Effect of Acetic Acid on Citric Acid Fermentation in an Integrated Citric Acid–Methane Fermentation Process. Appl Biochem Biotechnol 174, 376–387 (2014). https://doi.org/10.1007/s12010-014-1070-4

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