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Analysis of Genetic Diversity of Certain Species of Piper Using RAPD-Based Molecular Markers

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Abstract

The utility of RAPD markers in assessing genetic diversity and phenetic relationships of six different species of Piper from Northeast India was investigated. Polymerase chain reaction (PCR) with four arbitrary 10-mer oligonucleotide primers applied to the six species produced a total of 195 marker bands, of which, 159 were polymorphic. On average, six RAPD fragments were amplified per reaction. In the UPGMA phenetic dendrogram based on Jaccard’s coefficient, the different accessions of Piper showed a high level of genetic variation. This study may be useful in identifying diverse genetic stocks of Piper, which may then be conserved on a priority basis.

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Authors and Affiliations

  1. Department of Botany, B. Borooah College, Guwahati, Assam, India

    Utpal Chowdhury

  2. Department of Botany, Gauhati University, Assam, India

    Bhaben Tanti

  3. Department of Forestry, NERIST, Itanagar, Arunachal Pradesh, India

    Parakkal Rethy & Padma Raj Gajurel

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  1. Utpal Chowdhury
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  2. Bhaben Tanti
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  3. Parakkal Rethy
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  4. Padma Raj Gajurel
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Correspondence to Utpal Chowdhury.

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Chowdhury, U., Tanti, B., Rethy, P. et al. Analysis of Genetic Diversity of Certain Species of Piper Using RAPD-Based Molecular Markers. Appl Biochem Biotechnol 174, 168–173 (2014). https://doi.org/10.1007/s12010-014-1053-5

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  • DOI: https://doi.org/10.1007/s12010-014-1053-5

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