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Siderophore as a Potential Plant Growth-Promoting Agent Produced by Pseudomonas aeruginosa JAS-25

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Abstract

Siderophores scavenges Fe+3 from the vicinity of the roots of plants, and thus limit the amount of iron required for the growth of pathogens such as Fusarium oxysporum, Pythium ultimum, and Fusarium udum, which cause wilt and root rot disease in crops. The ability of Pseudomonas to grow and to produce siderophore depends upon the iron content, pH, and temperature. Maximum yield of siderophore of 130 μM was observed at pH 7.0 ± 0.2 and temperature of 30 °C at 30 h. The threshold level of iron was 50 μM, which increases up to 150 μM, favoring growth but drastically affecting the production of siderophore by Pseudomonas aeruginosa JAS-25. The seeds of agricultural crops like Cicer arietinum (chick pea), Cajanus cajan (pigeon pea), and Arachis hypogaea (ground nut) were treated with P. aeruginosa JAS-25, which enhanced the seed germination, root length, shoot length, and dry weight of chick pea, pigeon pea, and ground nut plants under pot studies. The efficient growth of the plants was not only due to the biocontrol activity of the siderophore produced by P. aeruginosa JAS-25 but also may be by the production of indole acetic acid (IAA), which influences the growth of the plants as phytohormones.

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Acknowledgments

Financial support (UGC no. F. no. 37-162/2009(SR)) by UGC, Government of India, Ministry of Science and Technology, New Delhi is highly acknowledged for granting UGC Major Research Project. Our sincere thanks to Dr. Yogesh S. Shouche, Scientist, National Center for Cell Sciences, Pune, Maharashtra, India for his kind help in the identification of the strain.

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Authors and Affiliations

  1. Department of PG Studies and Research in Biotechnology, Gulbarga University, Gulbarga, 585106, Karnataka, India

    M. B. Sulochana, S. Y. Jayachandra, S. Anil Kumar, A. B. Parameshwar & K. Mohan Reddy

  2. Department of PG Studies and Research in Microbiology, Gulbarga University, Gulbarga, 585106, India

    A. Dayanand

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  1. M. B. Sulochana
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  2. S. Y. Jayachandra
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Correspondence to M. B. Sulochana.

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Sulochana, M.B., Jayachandra, S.Y., Kumar, S.A. et al. Siderophore as a Potential Plant Growth-Promoting Agent Produced by Pseudomonas aeruginosa JAS-25. Appl Biochem Biotechnol 174, 297–308 (2014). https://doi.org/10.1007/s12010-014-1039-3

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  • DOI: https://doi.org/10.1007/s12010-014-1039-3

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