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Ferrocenylnaphthalene Diimide-Based Electrochemical Detection of Aberrant Methylation in hTERT Gene

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Abstract

Since aberrant methylation at CpG sites is linked to the silencing of tumor suppressor genes, DNA methylation analysis is important for cancer diagnosis. We developed ferrocenylnaphthalene diimide (FND), which has two ferrocenyl moieties at the substituent termini, as an electrochemical indicator for hybridized DNA duplexes. In this study, we attempted to detect aberrant methylation of human telomerase reverse transcriptase gene (hTERT), an efficient cancer marker, using FND-based hybridization coupled with electrochemical detection via a multi-electrode chip.

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Acknowledgments

This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan.

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Authors and Affiliations

  1. Department of Applied Chemistry, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu, Fukuoka, 804-8550, Japan

    Shinobu Sato, Toshiro Saeki, Tomoki Tanaka, Yusuke Kanezaki & Shigeori Takenaka

  2. Research Center for Biomicrosensing Technology, Kyushu Institute of Technology, 1-1 Sensui-cho, Tobata-ku, Kitakyushu, Fukuoka, 804-8550, Japan

    Shinobu Sato & Shigeori Takenaka

  3. Department of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan

    Mana Hayakawa, Kazuya Haraguchi, Masaaki Kodama & Kazuhiro Tominaga

  4. Department of Health Promotion, Division of Infections and Molecular Biology, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan

    Tatsuji Nishihara

  5. Oral Bioresearch Center, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyushu, Fukuoka, 803-8580, Japan

    Masaaki Kodama, Tatsuji Nishihara & Kazuhiro Tominaga

Authors
  1. Shinobu Sato
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  2. Toshiro Saeki
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  3. Tomoki Tanaka
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  4. Yusuke Kanezaki
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  5. Mana Hayakawa
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  6. Kazuya Haraguchi
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  7. Masaaki Kodama
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  8. Tatsuji Nishihara
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  9. Kazuhiro Tominaga
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  10. Shigeori Takenaka
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Corresponding author

Correspondence to Shigeori Takenaka.

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Sato, S., Saeki, T., Tanaka, T. et al. Ferrocenylnaphthalene Diimide-Based Electrochemical Detection of Aberrant Methylation in hTERT Gene. Appl Biochem Biotechnol 174, 869–879 (2014). https://doi.org/10.1007/s12010-014-1030-z

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  • DOI: https://doi.org/10.1007/s12010-014-1030-z

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