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Extractive Fermentation of Xylanase from Aspergillus tamarii URM 4634 in a Bioreactor

Applied Biochemistry and Biotechnology volume 173, pages 1652–1666 (2014)Cite this article

Abstract

Of the many reported applications for xylanase, its use as a food supplement has played an important role for monogastric animals, because it can improve the utilisation of nutrients. The aim of this work was to produce xylanase by extractive fermentation in an aqueous two-phase system using Aspergillus tamarii URM 4634, increasing the scale of production in a bioreactor, partially characterising the xylanase and evaluating its influence on monogastric digestion in vitro. Through extractive fermentation in a bioreactor, xylanase was obtained with an activity of 331.4 U mL−1 and 72 % yield. The xylanase was stable under variable pH and temperature conditions, and it was optimally active at pH 3.6 and 90 °C. Xylanase activity potentiated the simulation of complete monogastric digestion by 6 %, and only Mg2+ inhibited its activity. This process provides a system for efficient xylanase production by A. tamarii URM 4634 that has great potential for industrial use.

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Acknowledgments

The authors thank FACEPE, CNPq, and CAPES (PROCAD/NF 2009 N. 091/2010) by funding and encouraging research.

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Affiliations

  1. Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco, Av. Bom Pastor, s/n, Boa Vista, CEP 55.292-270, Garanhuns, PE, Brazil

    Anna Carolina da Silva, Alana Emília Soares de França Queiroz, Talita Camila Evaristo dos Santos Nascimento, José Erick Galindo Gomes, Erika Valente de Medeiros & Keila Aparecida Moreira

  2. Universidade Federal do Paraná/Centro Politécnico, Av. Coronel Francisco Heráclito dos Santos, 210-Jardim das Américas, CEP 81531-970, Curitiba, PR, Brazil

    Cristine Rodrigues & Luciana Porto de Souza Vandenberghe

  3. Departamento de Micologia, Universidade Federal de Pernambuco, Av. Moraes Rego, s/n, CEP 50670-901, Recife, PE, Brazil

    Cristina Maria Souza-Motta

  4. Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, CEP 52171-900, Recife, PE, Brazil

    Polyanna Nunes Herculano

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  1. Anna Carolina da Silva
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  2. Alana Emília Soares de França Queiroz
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Correspondence to Keila Aparecida Moreira.

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da Silva, A.C., Soares de França Queiroz, A.E., Evaristo dos Santos Nascimento, T.C. et al. Extractive Fermentation of Xylanase from Aspergillus tamarii URM 4634 in a Bioreactor. Appl Biochem Biotechnol 173, 1652–1666 (2014). https://doi.org/10.1007/s12010-014-0953-8

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  • DOI: https://doi.org/10.1007/s12010-014-0953-8

Keywords

  • Aqueous two-phase
  • Bioreactors
  • Filamentous fungi
  • Scale up
  • Xylanolytic complex
  • Monogastric digestion