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Optimization of Culture Conditions for Enhanced Lysine Production Using Engineered Escherichia coli

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Abstract

In this study, culture conditions, including dissolved oxygen (DO) content, presence of osmoprotectants, residual glucose concentration, and ammonium sulfate-feeding strategies, were investigated for decreasing the inhibition effects of acetic acid, ammonium, and osmotic stress on l-lysine fermentation by Escherichia coli. The results revealed that higher DO content and lower residual glucose concentration could decrease acetic acid accumulation, betaine supplementation could enhance osmotic stress tolerance, and variable speed ammonium sulfate-feeding strategy could decrease ammonium inhibition. Thus, with 25 % DO content, 0–5.0 g/L of residual glucose concentration, and 1.5 g/L of betaine supplementation, 134.9 g/L of l-lysine was obtained after 72 h of culture, with l-lysine yield and productivity of 45.4 % and 1.9 g/(L · h), respectively.

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Acknowledgments

This work was supported by the National Nature Science Foundation of China (grant no. 21106066, 21106068), National Key Technology Support Program (grant no. 2012BAI44G00), “973” program of China (grant no. 2011CBA00807), and Nature Science Foundation of Jiangsu High School (11KJB530003).

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  1. State Key Laboratory of Materials-Oriented Chemical Engineering College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, 211816, People’s Republic of China

    Hanxiao Ying, Xun He, Yan Li, Kequan Chen & Pingkai Ouyang

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  1. Hanxiao Ying
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  2. Xun He
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  3. Yan Li
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Correspondence to Kequan Chen.

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Ying, H., He, X., Li, Y. et al. Optimization of Culture Conditions for Enhanced Lysine Production Using Engineered Escherichia coli . Appl Biochem Biotechnol 172, 3835–3843 (2014). https://doi.org/10.1007/s12010-014-0820-7

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  • DOI: https://doi.org/10.1007/s12010-014-0820-7

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