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Antimicrobial Activity and Physical Characterization of Silver Nanoparticles Green Synthesized Using Nitrate Reductase from Fusarium oxysporum

Applied Biochemistry and Biotechnology volume 172, pages 4084–4098 (2014)Cite this article

Abstract

Nanostructures from natural sources have received major attention due to wide array of biological activities and less toxicity for humans, animals, and the environment. In the present study, silver nanoparticles were successfully synthesized using a fungal nitrate reductase, and their biological activity was assessed against human pathogenic fungi and bacteria. The enzyme was isolated from Fusarium oxysporum IRAN 31C after culturing on malt extract-glucose-yeast extract-peptone (MGYP) medium. The enzyme was purified by a combination of ultrafiltration and ion exchange chromatography on DEAE Sephadex and its molecular weight was estimated by gel filtration on Sephacryl S-300. The purified enzyme had a maximum yield of 50.84 % with a final purification of 70 folds. With a molecular weight of 214 KDa, it is composed of three subunits of 125, 60, and 25 KDa. The purified enzyme was successfully used for synthesis of silver nanoparticles in a way dependent upon NADPH using gelatin as a capping agent. The synthesized silver nanoparticles were characterized by X-ray diffraction, dynamic light scattering spectroscopy, and transmission and scanning electron microscopy. These stable nonaggregating nanoparticles were spherical in shape with an average size of 50 nm and a zeta potential of −34.3. Evaluation of the antimicrobial effects of synthesized nanoparticles by disk diffusion method showed strong growth inhibitory activity against all tested human pathogenic fungi and bacteria as evident from inhibition zones that ranged from 14 to 25 mm. Successful green synthesis of biologically active silver nanoparticles by a nitrate reductase from F. oxysporum in the present work not only reduces laborious downstream steps such as purification of nanoparticle from interfering cellular components, but also provides a constant source of safe biologically-active nanomaterials with potential application in agriculture and medicine.

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Acknowledgments

This work was supported financially by the Pasteur Institute of Iran (Grants Nos. 586 and 647).

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Authors and Affiliations

  1. Department of Mycology, Pasteur Institute of Iran, Tehran, 13164, Iran

    Mohammadhassan Gholami-Shabani & Mehdi Razzaghi-Abyaneh

  2. Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, 13164, Iran

    Mohammadhassan Gholami-Shabani, Azim Akbarzadeh, Dariush Norouzian, Afshin Imani & Mohsen Chiani

  3. Department of Biotechnology, Pasteur Institute of Iran, Tehran, 13164, Iran

    Abdolhossein Amini

  4. Faculty of Aerospace, Science and Research Campus, Islamic Azad University, Tehran, Iran

    Zeynab Gholami-Shabani

  5. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Gholamhossein Riazi

  6. Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Masoomeh Shams-Ghahfarokhi

Authors
  1. Mohammadhassan Gholami-Shabani
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  2. Azim Akbarzadeh
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  3. Dariush Norouzian
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  4. Abdolhossein Amini
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  5. Zeynab Gholami-Shabani
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  8. Gholamhossein Riazi
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Correspondence to Mehdi Razzaghi-Abyaneh.

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Gholami-Shabani, M., Akbarzadeh, A., Norouzian, D. et al. Antimicrobial Activity and Physical Characterization of Silver Nanoparticles Green Synthesized Using Nitrate Reductase from Fusarium oxysporum . Appl Biochem Biotechnol 172, 4084–4098 (2014). https://doi.org/10.1007/s12010-014-0809-2

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  • DOI: https://doi.org/10.1007/s12010-014-0809-2

Keywords

  • Fusarium oxysporum
  • Nitrate reductase
  • Green synthesis
  • Silver nanoparticles
  • Antimicrobial activity
  • Electron microscopy
  • Fungi
  • Bacteria