Skip to main content

Aqueous Extract of Crataegus azarolus Protects Against DNA Damage in Human Lymphoblast Cell K562 and Enhances Antioxidant Activity

Applied Biochemistry and Biotechnology volume 172, pages 2266–2275 (2014)Cite this article

Abstract

The present study was carried out to characterize the cellular antioxidant effect of the aqueous extract of Crataegus azarolus and its antigenotoxic potential using human myelogenous cells, K562. The antioxidant capacity of this extract was evaluated by determining its cellular antioxidant activity (CAA) in K562 cells. Also, preceding antigenotoxicity assessment, its eventual genotoxicity property was investigated by evaluating its capacity to induce the DNA degradation of treated cell nuclei. As no genotoxicity was detected at different exposure times, its ability to protect cell DNA against H2O2 oxidative effect was investigated, using the “comet assay.” It appears that 800 μg/mL of extract inhibited the genotoxicity induced by H2O2 with a rate of 41.30 %, after 4 h of incubation. In addition, this extract revealed a significant cellular antioxidant capacity against the reactive oxygen species in K562 cells.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Barnes, J., Anderson, L. A., & Phillipson, J. D. (2002). Herbal Medicines 2002. London: Pharmaceutical Press.

    Google Scholar 

  2. Bahorun, T., Aumjaud, E., Ramphul, H., Rycha, M., Luximon-Ramma, A., Trotin, F., et al. (2003). Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts. Nahrung, 47, 191–198.

    CAS  Article  Google Scholar 

  3. Chang, Q., Zuo, Z., Harrison, F., & Chow, M. S. (2002). Hawthorn. Journal of Clinical Pharmacology, 42, 605–612.

    Article  Google Scholar 

  4. Pottier-Alapetite, G. (1979). Flore de la Tunisia: Angiospermes, Dicotyledones, Apetales, Dialypetales. Tunis: Ministère de L'Enseignement Supérieur et de la Recherche Scientifique et Ministère de l'Agriculture.

    Google Scholar 

  5. Boukef, M.K. (1986). Les plantes dans la médecine traditionnelle tunisienne, médecine traditionnelle et pharmacopée. Paris: Agence de Coopération Culturelle et Technique.

  6. Aruoma, O. I. (1999). Antioxidant action of plant foods: use of oxidative DNA damage as a tool for studying antioxidant efficacy. Free Radical Research, 30, 419–427.

    CAS  Article  Google Scholar 

  7. Edwards, J. E., Brown, P. N., Talent, N., Dickinson, T. A., & Shipley, P. R. (2012). A review of the chemistry of the genus Crataegus. Phytochemistry, 79, 5–26.

    CAS  Article  Google Scholar 

  8. Scassellati-Sforzolini, G., Villarini, M., Moretti, M., Marcarelli, M., Pasquini, R., Fatigoni, C., et al. (1999). Antigenotoxic properties of Terminalia arjuna bark extracts. Journal of Environmental Pathology, Toxicology and Oncology, 18, 119–125.

    CAS  Google Scholar 

  9. Collins, A. R., Dusinska, M., Gedik, C. M., & Stetina, R. (1996). Oxidative damage to DNA: do we have a reliable biomarker? Environmental Health Perspectives, 104, 465–469.

    CAS  Google Scholar 

  10. Wolfe, K. L., & Liu, R. H. (2007). Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. Journal of Agricultural and Food Chemistry, 55, 8896–8907.

    CAS  Article  Google Scholar 

  11. Limem-Ben Amor, I., Skandrani, I., Boubaker, J., Ben Sghaier, M., Neffati, M. A., Bhouri, W., et al. (2009). Investigation of biological activity of polar extracts isolated from Phlomis crinita Cav ssp. mauritanica Munby. Drug and Chemical Toxicology, 32, 38–46.

    CAS  Article  Google Scholar 

  12. Skandrani, I., Boubaker, J., Bouhlel, I., Limem, I., Ghedira, K., & Chekir-Ghedira, L. (2010). Leaf and root extracts of Moricandia arvensis protect against DNA damage in human lymphoblast cell K562 and enhance antioxidant activity. Environmental Toxicology and Pharmacology, 30, 61–67.

    CAS  Article  Google Scholar 

  13. Ben Sghaier, M., Harizi, H., Louhichi, T., Krifa, M., Ghedira, K., & Chekir-Ghedira, L. (2011). Anti-inflammatory and antiulcerogenic activities of leaf extracts and sesquiterpene from Teucrium ramosissimum (Lamiaceae). Immunopharmacology and Immunotoxicology, 33, 656–662.

    Article  Google Scholar 

  14. Krifa, M., Bouhlel, I., Ghedira-Chekir, L., & Ghedira, K. (2013). Immunomodulatory and cellular anti-oxidant activities of an aqueous extract of Limoniastrum guyonianum gall. Journal of Ethnopharmacology, 146, 243–249.

    CAS  Article  Google Scholar 

  15. Wang, H., Du, Y. J., & Song, H. C. (2010). α-Glucosidase and α-amylase inhibitory activities of guava leaves. Food Chemistry, 123, 6–13.

    CAS  Article  Google Scholar 

  16. Piao, M. J., Kang, K. A., Zhang, R., Ko, D. O., Wang, Z. H., You, H. J., et al. (2008). Hyperoside prevents oxidative damage induced by hydrogen peroxide in lung fibroblast cells via an antioxidant effect. Biochimica et Biophysica Acta, 1780, 1448–1457.

    CAS  Article  Google Scholar 

  17. Zou, Y., Lu, Y., & Wei, D. (2004). Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. Journal of Agricultural and Food Chemistry, 52, 5032–5039.

    CAS  Article  Google Scholar 

  18. Kaur, P., Kaur, S., Kumar, N., Singh, B., & Kumar, S. (2009). Evaluation of antigenotoxic activity of isoliquiritin apioside from Glycyrrhiza glabra L. Toxicology in Vitro, 23, 680–686.

    CAS  Article  Google Scholar 

  19. Kumar, A., & Chattopadhyay, S. (2006). DNA damage protecting activity and antioxidant potential of pudina extract. Food Chemistry, 100, 1377–1384.

    Article  Google Scholar 

  20. Kun-Young, P., Geun-Ok, J., Kyung-Tae, L., Jongwon, C., Moo-Young, C., Gab-Tar, K., et al. (2004). Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua. Journal of Ethnopharmacology, 90, 73–79.

    Article  Google Scholar 

Download references

Conflict of Interest

No conflict of interest is declared.

Author information

Affiliations

  1. Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Médecine Dentaire, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisia

    Nadia Mustapha, Inès Bouhlel, Fadwa Chaabane, Imèn Mokdad Bzéouich & Leila Chekir-Ghedira

  2. Unité de Substances Naturelles Bioactives et Biotechnologie UR12ES12, Faculté de Pharmacie de Monastir, Université de Monastir, Rue Avicenne, 5000, Monastir, Tunisia

    Nadia Mustapha, Inès Bouhlel, Fadwa Chaabane, Imèn Mokdad Bzéouich, Kamel Ghedira & Leila Chekir-Ghedira

  3. Laboratoire de Pharmacognosie, E.A. 4481, Faculté de Pharmacie, Université de Lille 2, B.P. 83, 59006, Lille CEDEX, France

    Thierry Hennebelle

Authors
  1. Nadia Mustapha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Inès Bouhlel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fadwa Chaabane
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Imèn Mokdad Bzéouich
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kamel Ghedira
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Thierry Hennebelle
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Leila Chekir-Ghedira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Leila Chekir-Ghedira.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mustapha, N., Bouhlel, I., Chaabane, F. et al. Aqueous Extract of Crataegus azarolus Protects Against DNA Damage in Human Lymphoblast Cell K562 and Enhances Antioxidant Activity. Appl Biochem Biotechnol 172, 2266–2275 (2014). https://doi.org/10.1007/s12010-013-0667-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-013-0667-3

Keywords

  • Crataegus azarolus
  • Antigenotoxic activity
  • Comet assay
  • Cellular antioxidant activity
  • K562