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Poly(HEMA-co-NBMI) Monolithic Cryogel Columns for IgG Adsorption

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Abstract

Supermacroporous poly(2-hydroxyethyl methacrylate-co-1,5-naphthalene bismaleimide) [poly(HEMA-co-NBMI)] monolithic cryogel column was prepared by free radical cryo-copolymerization of HEMA with NBMI as a hydrophobic functional comonomer and N,N′-methylene-bisacrylamide as cross-linker directly in a plastic syringe for adsorption of albumin. The monolithic cryogel contained a continuous polymeric matrix which has interconnected pores of 10–100 μm size. Poly(HEMA-co-NBMI) cryogel was characterized by swelling studies, FTIR and scanning electron microscopy. The equilibrium swelling degree of the poly(HEMA-co-NBMI) cryogel was 10.5 g of H2O/g dry cryogel. Poly(HEMA-co-NBMI) cryogel was used in the adsorption/desorption of IgG from aqueous solutions. The maximum amount of IgG adsorption from aqueous solution in phosphate buffer was 98.20 mg/g polymer at pH 7.0. The nonspecific adsorption of IgG onto plain poly(HEMA) cryogel was very low (2.79 g/g polymer). It was observed that IgG could be repeatedly adsorbed and desorbed with the poly(HEMA-co-NBMI) cryogel without significant loss of adsorption capacity.

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Authors and Affiliations

  1. Koçarlı Vocational and Training School, Adnan Menderes University, Aydın, Turkey

    Murat Uygun

  2. Faculty of Science, Biochemistry Department, Ege University, Izmir, Turkey

    R. Hilal Şenay & Sinan Akgöl

  3. Ege Higher Vocational School, Ege University, 35100, Bornova, Izmir, Turkey

    Nesibe Avcıbaşı

Authors
  1. Murat Uygun
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  2. R. Hilal Şenay
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  3. Nesibe Avcıbaşı
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  4. Sinan Akgöl
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Correspondence to Nesibe Avcıbaşı.

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Uygun, M., Şenay, R.H., Avcıbaşı, N. et al. Poly(HEMA-co-NBMI) Monolithic Cryogel Columns for IgG Adsorption. Appl Biochem Biotechnol 172, 1574–1584 (2014). https://doi.org/10.1007/s12010-013-0624-1

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  • DOI: https://doi.org/10.1007/s12010-013-0624-1

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