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Production and Characteristics of a Bioflocculant by Klebsiella pneumoniae YZ-6 Isolated from Human Saliva

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Abstract

The production and characterization of a bioflocculant, MBF-6, by Klebsiella pneumoniae was investigated. Optimum culture conditions for bioflocculant production were an initial medium pH of 7, an incubation temperature of 30 °C, and an inoculum size of 1 % (v/v) of cell density 1.0 × 108 cfu/mL. The carbon, nitrogen, and cation sources for optimum bioflocculant production were glucose, peptone, and ZnCl2. The bioflocculant mainly consisted of protein (3.4 %) and sugar (95.1 %). Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl and hydroxyl groups while the thermogravimetric analysis (TGA) showed a degradation temperature (T d) of 81.4 °C. MBF-6 had a good flocculating rate in kaolin suspension without cation addition and was stable over a wide range of pH and temperature. Investigation on the flocculation efficacy of the characterized MBF-6 for wastewater treatment of dairy, woolen, brewery, and sugar industries suggested it to be effective.

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Acknowledgments

This study was supported by a research fund of Wuhan Textile University (grant no. 2013018) and China Postdoctoral Science Foundation funded project (No. 2013M541656).

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  1. Jiangang Zhou

    Present address: School of Environmental Engineering, Wuhan Textile University, FangZhi Road, Wuhan, 430073, China

Authors and Affiliations

  1. School of Environmental Engineering, Wuhan Textile University, FangZhi Road, Wuhan, 430073, China

    Zhengshan Luo, Changhong Chen, Wei Zhang, Ming Liu & Ye Han

  2. Central China Normal University Library, Wuhan, 430079, China

    Li Chen

Authors
  1. Zhengshan Luo
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  2. Li Chen
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  3. Changhong Chen
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  4. Wei Zhang
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  5. Ming Liu
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  6. Ye Han
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  7. Jiangang Zhou
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Correspondence to Jiangang Zhou.

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Zhengshan Luo and Li Chen contributed equally to this work.

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Luo, Z., Chen, L., Chen, C. et al. Production and Characteristics of a Bioflocculant by Klebsiella pneumoniae YZ-6 Isolated from Human Saliva. Appl Biochem Biotechnol 172, 1282–1292 (2014). https://doi.org/10.1007/s12010-013-0601-8

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