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Sensitive Electrochemical Aptasensor for Thrombin Detection Based on Graphene Served as Platform and Graphene Oxide as Enhancer

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Abstract

A sensitive electrochemical aptasensor was developed with conductive graphene served as platform and inert graphene oxide (GO) as enhancer. An electrodeposited nano-Au layer was firstly formed on conductive graphene modified glass carbon electrode surface for further immobilizing of electrochemical redox probe hexacyanoferrates nanoparticles (NiHCFNPs). Subsequently, another nano-Au layer was formed for immobilizing of thrombin aptamer (TBA). In the presence of thrombin, the TBA on the electrode surface could bind with thrombin, which made a barrier for electrons and inhibited the electro-transfer, resulting in the decreased electrochemical signals of NiHCFNPs. Owing to the non-conductivity property of graphene oxide, further decreased electrochemical signals of NiHCFNPs could be obtained via the sandwich reaction with GO-labeled TBA. According to the signal changes before the thrombin recognition and after sandwich reaction, trace detection of thrombin could be achieved. As a result, the proposed approach showed a high sensitivity and a wider linearity to thrombin in the range from 0.005 nM to 50 nM with a detection limit of 1 pM.

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Abbreviations

CVs:

Cyclic voltammetries

DMF:

Dimethylformamide

GO:

Graphene oxide

GCE:

Glass carbon electrode

NiHCFNPs:

Hexacyanoferrates nanoparticles

BSA:

Bovine serum albumin

TBA:

Thrombin aptamer

AuNPs:

Au nanoparticles

Hb:

Hemoglobin

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Acknowledgments

This work was supported by the Outstanding Youth Foundation of Yibin University (2009Q22).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Yibin University, Sichuan, 644000, China

    Chun He, Zenghong Xu, Tao Sun & Li Wang

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  1. Chun He
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  2. Zenghong Xu
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  3. Tao Sun
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  4. Li Wang
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Correspondence to Li Wang.

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He, C., Xu, Z., Sun, T. et al. Sensitive Electrochemical Aptasensor for Thrombin Detection Based on Graphene Served as Platform and Graphene Oxide as Enhancer. Appl Biochem Biotechnol 172, 1018–1026 (2014). https://doi.org/10.1007/s12010-013-0588-1

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  • DOI: https://doi.org/10.1007/s12010-013-0588-1

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