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Fungal Cellulase/Xylanase Production and Corresponding Hydrolysis Using Pretreated Corn Stover as Substrates

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Abstract

Three pretreated corn stover (ammonia fiber expansion, dilute acid, and dilute alkali) were used as carbon source to culture Trichoderma reesei Rut C-30 for cellulase and xylanase production. The results indicated that the cultures on ammonia fiber expansion and alkali pretreated corn stover had better enzyme production than the acid pretreated ones. The consequent enzymatic hydrolysis was performed applying fungal enzymes on pretreated corn stover samples. Tukey’s statistical comparisons exhibited that there were significant differences on enzymatic hydrolysis among different combination of fungal enzymes and pretreated corn stover. The higher sugar yields were achieved by the enzymatic hydrolysis of dilute alkali pretreated corn stover.

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References

  1. Huber, G. W., Iborra, S., & Corma, A. (2006). Chemical Reviews, 106, 4044–4098.

    Article  CAS  Google Scholar 

  2. Yue, Z., Teater, C., Liu, Y., Maclellan, J., & Liao, W. (2010). Biotechnology and Bioengineering, 105, 1031–1039.

    CAS  Google Scholar 

  3. Hofrichter, M. (2002). Enzyme and Microbial Technology, 30, 454–466.

    Article  CAS  Google Scholar 

  4. Ruan, Z., Zanotti, M., Zhong, Y., Liao, W., Ducey, C., & Liu, Y. (2013). Biotechnology and Bioengineering, 110, 1039–1049.

    Article  CAS  Google Scholar 

  5. Perlack, R. D., Wright, L. L., Turhollow, A. F., Graham, R. L., Stokes, B. J., & Erbach, D. C. (2005). OAK RIDGE NATIONAL LAB TN.

  6. Zheng, Y., Pan, Z., Zhang, R., Labavitch, J. M., Wang, D., & Teter, S. A. (2007). Applied Biochemistry and Biotechnology, 136–140, 423–435.

    Article  Google Scholar 

  7. Sun, Y., & Cheng, J. (2002). Bioresource Technology, 83, 1–11.

    Article  CAS  Google Scholar 

  8. Fang, H., Zhao, C., & Song, X. Y. (2010). Bioresource Technology, 101, 4111–4119.

    Article  CAS  Google Scholar 

  9. Linko, S. (1992). Biotechnology Advances, 10, 191–236.

    Article  CAS  Google Scholar 

  10. Mandels, M., & Weber, J. (1969). Advances in Chemistry Series, 95, 391–414.

    Article  CAS  Google Scholar 

  11. Bigelow, M., & Wyman, C. E. (2002). Applied Biochemistry and Biotechnology, 98–100, 921–934.

    Article  Google Scholar 

  12. Cheng, Y., Song, X., Qin, Y., & Qu, Y. (2009). Journal of Applied Microbiology, 107, 1837–1846.

    Article  CAS  Google Scholar 

  13. Szengyel, Z., Zacchi, G., Varga, A., & Reczey, K. (2000). Applied Biochemistry and Biotechnology, 84–86, 679–691.

    Article  Google Scholar 

  14. Lee, Y. H., & Fan, L. T. (1982). Biotechnology and Bioengineering, 24, 2383–2406.

    Article  CAS  Google Scholar 

  15. Lo, C. M., Zhang, Q., Callow, N. V., & Ju, L. K. (2010). Bioresource Technology, 101, 717–723.

    Article  CAS  Google Scholar 

  16. Zhang, L., Liu, Y., Niu, X., Liu, Y., & Liao, W. (2012). Biomass & Bioenergy, 37, 16–24.

    Article  Google Scholar 

  17. Ghose, T. (1987). Pure and Applied Chemistry, 59, 257–268.

    Article  CAS  Google Scholar 

  18. Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., & Templeton, D.(2008). Laboratory Analytical Procedure.

  19. Huang, A. A. (1975). Biotechnology and Bioengineering, 17, 1421–1433.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was financially supported by the Northeast Sun Grant Initiative, US Department of Transportation (grant number NE08-013), and the Program of Introducing Talents of Discipline to Universities, the State Administration of Foreign Experts Affairs, P. R. China (grant number 111-2-06). The authors would like to thank Dr. Bruce Dale at Michigan State University for providing AFEX corn stover samples and Genencor Inc. for providing Accellerase 1500 samples.

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Authors and Affiliations

  1. Department of Biosystems and Agricultural Engineering, Michigan State University, 203 Farrall Hall, East Lansing, MI, 48864, USA

    Liang Zhang, Xiaoqing Wang, Zhenhua Ruan, Ying Liu, Xiaorui Niu, Zhengbo Yue, Wei Liao & Yan Liu

  2. School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, China

    Liang Zhang

  3. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai, 200237, China

    Zhimin Li

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  1. Liang Zhang
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  2. Xiaoqing Wang
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  3. Zhenhua Ruan
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  4. Ying Liu
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  5. Xiaorui Niu
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  8. Wei Liao
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  9. Yan Liu
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Correspondence to Yan Liu.

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Zhang, L., Wang, X., Ruan, Z. et al. Fungal Cellulase/Xylanase Production and Corresponding Hydrolysis Using Pretreated Corn Stover as Substrates. Appl Biochem Biotechnol 172, 1045–1054 (2014). https://doi.org/10.1007/s12010-013-0584-5

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  • DOI: https://doi.org/10.1007/s12010-013-0584-5

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