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Reversible Immobilization of Urease by Using Bacterial Cellulose Nanofibers

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Abstract

In this work, bacterial cellulose nanofibers were produced by using the Gluconacetobacter hansenii HE1 strain. These nanofibers were derivatized with dye affinity ligand Reactive Green 5, and these newly synthesized dye-attached nanofibers were used for affinity adsorption of urease. Reactive Green 5-attached nanofibers were characterized by Fourier transform infrared spectroscopy, SEM, and energy-dispersive x-ray spectroscopy analysis. Some adsorption conditions which significantly affect the adsorption efficiency were investigated. The maximum urease adsorption capacity was found to be 240 mg/g nanofiber in pH 6.0 and at room temperature. Dye-free plain nanofibers also used for studying nonspecific urease adsorption onto plain nanofibers and nonspecific adsorption were found to be negligible (3.5 mg/g nanofiber). Prepared dye-attached nanofibers can be used in five successive adsorption/desorption steps without any decrease in their urease adsorption capacity. The desorption rate of the adsorbed urease was found to be 98.9 %. The activity of the urease was also investigated, and it was found that free and desorbed urease from the dye-attached nanofibers showed similar specific activity.

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Authors and Affiliations

  1. Chemistry Department, Adnan Menderes University, Aydın, Turkey

    Begüm Akduman & Deniz Aktaş Uygun

  2. Koçarlı Vocational and Training School, Adnan Menderes University, Aydın, Turkey

    Murat Uygun

  3. Biology Department, Adnan Menderes University, Aydın, Turkey

    Esin Poyrazoğlu Çoban & Halil Bıyık

  4. Biochemistry Department, Ege University, İzmir, Turkey

    Sinan Akgöl

Authors
  1. Begüm Akduman
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  2. Murat Uygun
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  3. Esin Poyrazoğlu Çoban
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  4. Deniz Aktaş Uygun
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  5. Halil Bıyık
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  6. Sinan Akgöl
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Correspondence to Deniz Aktaş Uygun.

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Akduman, B., Uygun, M., Çoban, E.P. et al. Reversible Immobilization of Urease by Using Bacterial Cellulose Nanofibers. Appl Biochem Biotechnol 171, 2285–2294 (2013). https://doi.org/10.1007/s12010-013-0541-3

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  • DOI: https://doi.org/10.1007/s12010-013-0541-3

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