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Characterization of a Layered Methylene Blue/Vanadium Oxide Nanocomposite and its Application in a Reagentless H2O2 Biosensor

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Abstract

Layered nanocomposite of methylene blue (MB)-intercalated vanadium oxide was obtained through a simple hydrothermal synthesis method using MB, V2O5, and NaI as starting materials. The intercalation reaction was proven to be successful using X-ray diffraction pattern. The MB-V2O5 nanocomposite was characterized using a scanning electron micrograph, infrared spectra, thermogravimetric analysis, UV spectra, and electrochemical measurements. The intercalated MB cations showed a fine diffusion-controlled electrochemical redox process and facilitated the immobilized horseradish peroxidase’s (HRP) good catalytic reduction upon H2O2. The as-prepared MB-V2O5/HRP biosensor showed a linear response to H2O2 over a range from 2.0 × 10−6 to 9.5 × 10−5 M with a detection limit of 9.7 × 10−7 M (S/N ratio = 3).

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Acknowledgments

This work was supported by Natural Science Fund of Jiangsu Province (BK2012665), Key University Science Research Project of Jiangsu Province (11KJA430008), and Jiangsu Marine Resource Development Research Institute Foundation (JSIMR10E06). This work is also funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions and Natural Science Foundation (Huaihai Institute of Technology, China, no. 2010150013).

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Authors and Affiliations

  1. Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang, 222005, People’s Republic of China

    Xiaobo Zhang, Dan Li, Fujun Yin, Junyan Gong & Zhiwei Tong

  2. Key Laboratory of Soft Chemistry and Functional Materials, Ministry of Education, Nanjing University of Science and Technology, Nanjing, 210094, People’s Republic of China

    Xiaobo Zhang, Fujun Yin, Xujie Yang & Xingyou Xu

  3. SORST, Japan Science and Technology Agency (JST), Kawaguchi Center Building 4-1-8, Kawaguchi-shi, Saitama, 332-0012, Japan

    Zhiwei Tong

  4. School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huai’an, 223003, People’s Republic of China

    Xingyou Xu

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  1. Xiaobo Zhang
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  2. Dan Li
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  3. Fujun Yin
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Correspondence to Zhiwei Tong.

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Zhang, X., Li, D., Yin, F. et al. Characterization of a Layered Methylene Blue/Vanadium Oxide Nanocomposite and its Application in a Reagentless H2O2 Biosensor. Appl Biochem Biotechnol 172, 176–187 (2014). https://doi.org/10.1007/s12010-013-0528-0

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  • DOI: https://doi.org/10.1007/s12010-013-0528-0

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