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Highly Thermostable and pH-Stable Cellulases from Aspergillus niger NS-2: Properties and Application for Cellulose Hydrolysis

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Abstract

Optimization of cultural conditions for enhanced cellulase production by Aspergillus niger NS-2 were studied under solid-state fermentation. Significant increase in yields (CMCase 463.9 ± 20.1 U/g, FPase 101.1 ± 3.5 U/g and β-glucosidase 99 ± 4.0 U/g) were obtained under optimized conditions. Effect of different nutritional parameters was studied to induce the maximum production of cellulase complex. Scale-up studies for enzyme production process were carried out. Characterization studies showed that enzymes produced by A. niger NS-2 were highly temperature- and pH stable. At 50 °C, the half life for CMCase, FPase, β-glucosidase were approximately 240 h. Cellulases from A. niger NS-2 were stable at 35 °C for 24 h over a broader pH range of 3.0–9.0. We examined the feasibility of using steam pretreatment to increase the saccharification yields from various lignocellulosic residues for sugar release which can potentially be used in bioethanol production. Saccharification of pretreated dry potato peels, carrot peels, composite waste mixture, orange peels, onion peels, banana peels, pineapple peels by crude enzyme extract from A. niger NS-2, resulted in very high cellulose conversion efficiencies of 92–98 %.

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Acknowledgments

This work was supported by University Grants Commission, New Delhi, under the special assistance programme (SAP) and by the Department of Science & Technology, Government of India under PURSE grant.

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Author notes

  1. Namita Bansal

    Present address: U.S. Department of Energy Great Lake Bioenergy Research Centre, Michigan State University, East Lansing, 48824, USA

Authors and Affiliations

  1. Department of Microbiology, Panjab University, Chandigarh, 160014, India

    Namita Bansal, Chetna Janveja & Sanjeev Kumar Soni

  2. Department of Biotechnology, Panjab University, Chandigarh, 160014, India

    Namita Bansal

  3. Centre for Microbial Biotechnology, Panjab University, Chandigarh, 160014, India

    Rupinder Tewari

  4. Department of Biotechnology, D.A.V. College, Chandigarh, 160011, India

    Raman Soni

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  1. Namita Bansal
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  5. Sanjeev Kumar Soni
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Correspondence to Sanjeev Kumar Soni.

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Bansal, N., Janveja, C., Tewari, R. et al. Highly Thermostable and pH-Stable Cellulases from Aspergillus niger NS-2: Properties and Application for Cellulose Hydrolysis. Appl Biochem Biotechnol 172, 141–156 (2014). https://doi.org/10.1007/s12010-013-0511-9

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