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Comparative Antioxidant Study of Stem and Stem Induced Callus of Phyllanthus fraternus Webster—An Important Antiviral and Hepatoprotective Plant

Applied Biochemistry and Biotechnology volume 171, pages 2153–2164 (2013)Cite this article

Abstract

Phyllanthus fraternus is widely used in the cure of various liver diseases and possess antiviral properties especially against hepatitis virus. In the present study, evaluation of the antioxidant activity of stem and calli induced from stem has been done by different assays. Extraction was done by standard method in water and ethanol. Total antioxidant capacity was measured by 1, 1-diphenyl-2-picrylhydrazyl free radical scavenging method. Lipid peroxidation was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg yolk homogenates as lipid-rich media, and superoxide radical scavenging activity was measured using riboflavin–light–nitro blue tetrazolium assay. Reducing power was determined on the basis of Fe3+–Fe2+ transformation in the presence of the extract. In addition to the antioxidant activity, polyphenolic compounds like total phenolics and flavonoids were also measured by spectroscopic method. Results showed that the ethanolic extract of stem is more potent in antioxidant activity than its aqueous extract and ethanolic extract of calli. A significant correlation between antioxidant capacity and polyphenolic content and reducing potential was observed, indicating that phenolic compounds and reducers present in extract are major contributors to the antioxidant potential. Thus, this plant extract could be used as a potent natural antioxidant.

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Abbreviations

ROS:

Reactive oxygen species

DPPH:

1, 1-Diphenyl-2-picrylhydrazyl

TP:

Total phenolics

TF:

Total flavonoids

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Acknowledgments

R. Upadhyay is highly thankful to the Council of Scientific and Industrial Research (CSIR), New Delhi, for providing fellowship as senior research fellow.

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Affiliations

  1. Department of Botany, MMV, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India

    Richa Upadhyay & Kavindra Nath Tiwari

  2. Department of Applied Science, Kashi Institute of Technology, Varanasi, 221307, Uttar Pradesh, India

    Jitendra Kumar Chaurasia

  3. Department of Zoology, MMV, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India

    Karuna Singh

Authors
  1. Richa Upadhyay
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  2. Jitendra Kumar Chaurasia
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  3. Kavindra Nath Tiwari
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  4. Karuna Singh
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Correspondence to Kavindra Nath Tiwari.

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Upadhyay, R., Chaurasia, J.K., Tiwari, K.N. et al. Comparative Antioxidant Study of Stem and Stem Induced Callus of Phyllanthus fraternus Webster—An Important Antiviral and Hepatoprotective Plant. Appl Biochem Biotechnol 171, 2153–2164 (2013). https://doi.org/10.1007/s12010-013-0487-5

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  • DOI: https://doi.org/10.1007/s12010-013-0487-5

Keywords

  • Phyllanthus
  • Callus
  • Antioxidant activity
  • Reducing power