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A Novel Polysaccharide from Lentinus edodes Mycelia Exhibits Potential Antitumor Activity on Laryngeal Squamous Cancer Cell Line Hep-2

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Abstract

A novel polysaccharide [Lentinus edodes mycelia polysaccharide 2 (LMP2)] with a molecular weight of 2.27 × 104 Da was isolated from the Lentinus edodes mycelia and purified by Sephadex G-200 and diethylaminoethyl-32 column chromatography. HPLC results indicated that LMP2 contained mannose, arabinose, galactose, xylose, and rhamnose with a relative molar ratio of 1:0.74:3.23:1.18:10.98. Its antitumor activity was evaluated in vitro by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, and transwell assay. LMP2 showed a remarkable inhibitory effect on the proliferation of Hep-2 cells, and at the concentration of 200 mg/ml, the inhibition ratio was 37.2 % after 72 h. Furthermore, colony formation of Hep-2 cells was reduced significantly after treatment with LMP2. Invasion of Hep-2 cells was inhibited significantly by LMP2. These results suggested that LMP2 could be explored as a potential antitumor material for laryngeal carcinoma.

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References

  1. Zhang, M., Cui, S. W., Cheung, P. C. K., & Wang, Q. (2007). Trends in Food Science & Technology, 18, 4–19.

    Article  Google Scholar 

  2. Ikekawa, T., Ueharam, N., Maedam, Y., Nakanishi, M., & Fukuoka, F. (1969). Cancer Research, 29, 734–735.

    CAS  Google Scholar 

  3. White, R. W. D., Hackman, R. M., Soares, S. E., Beckett, L. A., & Sun, B. (2002). Urology, 60, 640–644.

    Article  Google Scholar 

  4. Smith, J. E., Rowan, N. J., & Sullivan, R. (2002). Biotechnology Letters, 24, 1845–1938.

    Article  Google Scholar 

  5. Gao, T. T., Bi, H. T., Ma, S., & Lu, J. M. (2010). International Journal of Biological Macromolecules, 46, 85–90.

    Article  CAS  Google Scholar 

  6. Zong, A., Cao, H., & Wang, F. (2012). Carbohydrate Polymers, 90, 1395–1410.

    Article  CAS  Google Scholar 

  7. Liu, J., Guo, Y., Fu, S., Yang, M., Sun, K. L., & Fu, W. N. (2010). Oncology Reports, 23, 1101–1107.

    Article  CAS  Google Scholar 

  8. Finimundy, T. C., Gambato, G., Fontana, R., Camassola, M., Salvador, M., Moura, S., Hess, J., Henriques, J. A., Dillon, A. J., & Roesch-Ely, M. (2012). Nutrition Research, 33, 76–84.

    Article  Google Scholar 

  9. Tomi, H., Tamesada, M., Kawanishi, T., Ishikawa, S., & Yukawa, H. (2012). Biological & Pharmaceutical Bulletin, 35, 1014–1021.

    Article  Google Scholar 

  10. Saltarelli, R., Ceccaroli, O., Iotti, M., Zambonelli, A., Buffalini, M., Casadei, L., Vallorani, L., & Stocchi, V. (2009). Food Chemistry, 116, 143–151.

    Article  CAS  Google Scholar 

  11. Thetsrimuanga, C., Khammuang, S., Chiablaem, K., Srisomsap, C., & Sarnthim, R. (2011). Food Chemistry, 128, 634–639.

    Article  Google Scholar 

  12. Lee, B. C., Bae, J. T., Pyo, H. B., Choe, T. B., Kim, S. W., Hwang, H. J., & Jong, W. Y. (2003). Enzyme and Microbial Technology, 32, 574–581.

    Article  CAS  Google Scholar 

  13. Jeff, I. B., Yuan, X., Sun, L., Kassim, R. M., Foday, A. D., & Zhou, Y. (2013). International Journal of Biological Macromolecules, 52, 99–106.

    Article  CAS  Google Scholar 

  14. Zhang, Z., Wang, X., Yu, S., & Zhao, M. (2011). International Journal of Biological Macromolecules, 49, 454–457.

    Article  CAS  Google Scholar 

  15. Stepan, H., & Staudacher, E. (2011). Analytical Biochemistry, 418, 24–29.

    Article  CAS  Google Scholar 

  16. Liu, X., Sun, Z., Zhang, M., Meng, X., Xia, X., Yuan, W., Xue, F., & Liu, C. (2012). Carbohydrate Polymers, 90, 1664–1670.

    Article  CAS  Google Scholar 

  17. Cao, W., Li, X. Q., Wang, X., Fan, H. T., Zhang, X. N., Hou, Y., Liu, S. B., & Mei, Q. B. (2010). Phytomedicine, 17, 598–605.

    Article  CAS  Google Scholar 

  18. Mao, H., Zhang, L., Yang, Y., Sun, J., Deng, B., Feng, J., Shao, Q., Feng, A., Song, B., & Qu, X. (2011). Gene, 486, 74–80.

    Article  CAS  Google Scholar 

  19. Chen, D., Yao, W. J., Zhang, X. L., Han, X. Q., Qu, X. Y., Ka, W. B., Sun, D. G., Wu, X. Z., & Wen, Z. Y. (2010). Journal of Ethnopharmacology, 127, 702–708.

    Article  CAS  Google Scholar 

  20. Zhang, Y., Gu, M., Wang, K. P., Chen, Z. X., Dai, L. Q., Liu, J. Y., & Zeng, F. (2010). Fitoterapia, 81, 1163–1170.

    Article  CAS  Google Scholar 

  21. Wang, Y. Y., Khoo, K. H., Chen, S. T., Lin, C. C., Wong, C. H., & Lin, C. H. (2002). Bioorganic & Medicinal Chemistry, 10, 1057–1062.

    Article  CAS  Google Scholar 

  22. Liu, J. J., Huang, T. S., Hsu, M. L., Chen, C. C., Lin, W. S., Lu, F. J., & Chang, W. H. (2004). Toxicology and Applied Pharmacology, 201, 186–193.

    Article  CAS  Google Scholar 

  23. Kim, Y. O., Park, H. W., Kim, J. H., Lee, J. Y., Moon, S. H., & Shin, C. S. (2006). Life Science, 79, 72–80.

    Article  CAS  Google Scholar 

  24. He, N. W., Yang, X. B., Jiao, Y. D., Tian, L. M., & Zhao, Y. (2012). Food Chemistry, 133, 978–989.

    Article  CAS  Google Scholar 

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (no. 31240005), the Agriculture research Program of Science and Technology Department of Liaoning Province, China (no. 2011211001 and no. 2009209001), and the Program for Liaoning Excellent Talents in University (no. LJQ2013002), Startup Foundation for Doctors of Liaoning University.

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Authors and Affiliations

  1. School of Life Science, Liaoning University, Shenyang, 110036, China

    Xiangyu Cao, Jianli Liu, Yapeng Huo & Wei Yang

  2. State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, 300071, China

    Ruihua Liu

  3. Tianjin Academy of Environmental Sciences, Tianjin, 300191, China

    Meng Zeng

  4. Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China

    Chao Yang

Authors
  1. Xiangyu Cao
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  2. Ruihua Liu
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  3. Jianli Liu
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  4. Yapeng Huo
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  5. Wei Yang
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  6. Meng Zeng
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  7. Chao Yang
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Corresponding authors

Correspondence to Jianli Liu or Chao Yang.

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Xiangyu Cao and Ruihua Liu contributed equally to this work.

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Cao, X., Liu, R., Liu, J. et al. A Novel Polysaccharide from Lentinus edodes Mycelia Exhibits Potential Antitumor Activity on Laryngeal Squamous Cancer Cell Line Hep-2. Appl Biochem Biotechnol 171, 1444–1453 (2013). https://doi.org/10.1007/s12010-013-0441-6

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  • DOI: https://doi.org/10.1007/s12010-013-0441-6

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