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Biodegradable Polycaprolactone (PCL) Nanosphere Encapsulating Superoxide Dismutase and Catalase Enzymes

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Abstract

Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase (SOD) and catalase (CAT) were successfully synthesized using double emulsion (w/o/w) solvent evaporation technique. Characterization of the nanosphere using dynamic light scattering, field emission scanning electron microscope, and Fourier transform infrared spectroscopy revealed a spherical-shaped nanosphere in a size range of 812 ± 64 nm with moderate protein encapsulation efficiency of 55.42 ± 3.7 % and high in vitro protein release. Human skin HaCat cells were used for analyzing antioxidative properties of SOD- and CAT-encapsulated PCL nanospheres. Oxidative stress condition in HaCat cells was optimized with exposure to hydrogen peroxide (H2O2; 1 mM) as external stress factor and verified through reactive oxygen species (ROS) analysis using H2DCFDA dye. PCL nanosphere encapsulating SOD and CAT together indicated better antioxidative defense against H2O2-induced oxidative stress in human skin HaCat cells in comparison to PCL encapsulating either SOD or CAT alone as well as against direct supplement of SOD and CAT protein solution. Increase in HaCat cells SOD and CAT activities after treatment hints toward uptake of PCL nanosphere into the human skin HaCat cells. The result signifies the role of PCL-encapsulating SOD and CAT nanosphere in alleviating oxidative stress.

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Acknowledgments

Ph.D. fellowship to SS and ANS by Indian Institute of Technology Guwahati is acknowledged. Facilities at Department of Biotechnology and Central Instrument Facility, IIT Guwahati are acknowledged. Infrastructure facility by IIT Guwahati is also acknowledged.

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Authors and Affiliations

  1. Centre for Environment, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India

    Sushant Singh, Anil Verma & Vikash Kumar Dubey

  2. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India

    Abhay Narayan Singh & Vikash Kumar Dubey

  3. Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India

    Anil Verma

Authors
  1. Sushant Singh
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  2. Abhay Narayan Singh
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  3. Anil Verma
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  4. Vikash Kumar Dubey
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Correspondence to Vikash Kumar Dubey.

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Singh, S., Singh, A.N., Verma, A. et al. Biodegradable Polycaprolactone (PCL) Nanosphere Encapsulating Superoxide Dismutase and Catalase Enzymes. Appl Biochem Biotechnol 171, 1545–1558 (2013). https://doi.org/10.1007/s12010-013-0427-4

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  • DOI: https://doi.org/10.1007/s12010-013-0427-4

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