Abstract
We previously found that some Saccharomyces cerevisiae and Lactobacillus plantarum remarkably formed mixed-specie biofilm in a static co-culture and deduced that this biofilm had potential as immobilized cells. We investigated the application of mixed-specie biofilm formed by S. cerevisiae BY4741 and L. plantarum HM23 for ethanol fermentation in repeated batch cultures. This mixed-specie biofilm was far abundantly formed and far resistant to washing compared with S. cerevisiae single biofilm. Adopting mixed-specie biofilm formed on cellulose beads as immobilized cells, we could produce enough ethanol from 10 or 20 % glucose during ten times repeated batch cultures for a duration of 10 days. Cell numbers of S. cerevisiae and L. plantarum during this period were stable. In mixed-specie biofilm system, though ethanol production was slightly lower compared to S. cerevisiae single-culture system due to by-production of lactate, pH was stably maintained under pH 4 without artificial control suggesting high resistance to contamination. Inoculated model contaminants, Escherichia coli and Bacillus subtilis, were excluded from the system in a short time. From the above results, it was indicated that the mixed-specie biofilm of S. cerevisiae and L. plantarum was a promising immobilized cell for ethanol fermentation for its ethanol productivity and robustness due to high resistance to contamination.
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Acknowledgments
This work was supported by a Grant-in-Aid for Scientific Research (Wakate B & Kiban C) to SF from MEXT; the Ministry of Education, Culture, Sports, Science and Technology of Japan; High-Tech Research Center Project for Private Universities; Matching Fund Subsidy to SF; and MEXT and Adaptable and Seamless Technology Transfer Program through target drive R&D to YM from Japan Science and Technology Agency.
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Abe, A., Furukawa, S., Watanabe, S. et al. Yeasts and Lactic Acid Bacteria Mixed-Specie Biofilm Formation is a Promising Cell Immobilization Technology for Ethanol Fermentation. Appl Biochem Biotechnol 171, 72–79 (2013). https://doi.org/10.1007/s12010-013-0360-6
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DOI: https://doi.org/10.1007/s12010-013-0360-6