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Synergistic Antibacterial and Antibiofilm Effect Between (+)-Medioresinol and Antibiotics In Vitro

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Abstract

In this study, antibacterial effects of (+)-Medioresinol isolated from stem bark of Sambucus williamsii and its synergistic activities in combination with antibiotics such as ampicillin, cefotaxime, and chloramphenicol were tested by antibacterial susceptibility testing and checkerboard assay. (+)-Medioresinol possessed antibacterial effects against antibiotics-susceptible- or antibiotics-resistant strains. Most of combinations between (+)-Medioresinol and each antibiotic showed synergistic interaction (fractional inhibitory concentration index ≤0.5) against bacterial strains including antibiotics-resistant Pseudomonas aeruginosa. Furthermore, the antibiofilm effect of (+)-Medioresinol alone or in combination with each antibiotic was investigated. The results indicated that not only (+)-Medioresinol but also its combination with each antibiotic had antibiofilm activities. It concludes that (+)-Medioresinol has potential as a therapeutic agent and adjuvant for treatment of bacterial infection.

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Acknowledgments

This work was supported by the National Research Foundation of Korea grant funded by the Korea government (MEST) (no. 2008-0062618).

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daehak-ro 80, Buk-gu, Daegu, 702-701, Republic of Korea

    Ji Hong Hwang, Hyemin Choi, In-sok Hwang & Dong Gun Lee

  2. College of Pharmacy, Chosun University, Gwangju, 501-759, Republic of Korea

    A. Ryun Kim & Eun-Rhan Woo

Authors
  1. Ji Hong Hwang
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  2. Hyemin Choi
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  3. In-sok Hwang
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  4. A. Ryun Kim
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  5. Eun-Rhan Woo
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  6. Dong Gun Lee
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Correspondence to Dong Gun Lee.

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Ji Hong Hwang and Hyemin Choi contributed equally to this work and should be considered co-first authors.

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Hwang, J.H., Choi, H., Hwang, Is. et al. Synergistic Antibacterial and Antibiofilm Effect Between (+)-Medioresinol and Antibiotics In Vitro. Appl Biochem Biotechnol 170, 1934–1941 (2013). https://doi.org/10.1007/s12010-013-0351-7

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  • DOI: https://doi.org/10.1007/s12010-013-0351-7

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