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Production and Properties of Two Novel Exopolysaccharides Synthesized by a Thermophilic Bacterium Aeribacillus pallidus 418

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Abstract

Synthesis of innovative exocellular polysaccharides (EPSs) was reported for few thermophilic microorganisms as one of the mechanisms for surviving at high temperature. Thermophilic aerobic spore-forming bacteria able to produce exopolysaccharides were isolated from hydrothermal springs in Bulgaria. They were referred to four species, such as Aeribacillus pallidus, Geobacillus toebii, Brevibacillus thermoruber, and Anoxybacillus kestanbolensis. The highest production was established for the strain 418, whose phylogenetic and phenotypic properties referred it to the species A. pallidus. Maltose and NH4Cl were observed to be correspondingly the best carbon and nitrogen sources and production yield was increased more than twofold in the process of culture condition optimization. After purification of the polymer fraction, a presence of two different EPSs, electroneutral EPS 1 and negatively charged EPS 2, in a relative weight ratio 3:2.2 was established. They were heteropolysaccharides consisting of unusual high variety of sugars (six for EPS 1 and seven for EPS 2). Six of the sugars were common for both EPSs. The main sugar in EPS 1 was mannose (69.3 %); smaller quantities of glucose (11.2 %), galactosamine (6.3 %), glucosamine (5.4 %), galactose (4.7 %), and ribose (2.9 %) were also identified. The main sugar in EPS 2 was also mannose (33.9 %), followed by galactose (17.9 %), glucose (15.5 %), galactosamine (11.7 %), glucosamine (8.1 %), ribose (5.3 %), and arabinose (4.9 %). Both polymers showed high molecular weight and high thermostability.

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Acknowledgments

The authors are grateful to the National Fund for Scientific Research, Bulgaria for financial support of this work (Contract DTK 02/46).

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Authors and Affiliations

  1. Institute of Microbiology, Bulgarian Academy of Sciences, Acad G. Bonchev Str. Bl. 26, 1113, Sofia, Bulgaria

    Nadja Radchenkova, Spasen Vassilev, Iva Tomova & Margarita Kambourova

  2. University of Food Technologies, 26 Maritza Blvd., 4002, Plovdiv, Bulgaria

    Ivan Panchev & Margarita Kuncheva

  3. Institute of Biomolecular Chemistry ICB-CNR, Via Campi Flegrei, 34, 80078, Pozzuoli (NA), Italy

    Gianluca Anzelmo & Barbara Nicolaus

  4. Institute of Chemical Engineering, Acad. G. Bonchev Str. Bl. 103, 1113, Sofia, Bulgaria

    Kaloyan Petrov

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  1. Nadja Radchenkova
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Correspondence to Margarita Kambourova.

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Radchenkova, N., Vassilev, S., Panchev, I. et al. Production and Properties of Two Novel Exopolysaccharides Synthesized by a Thermophilic Bacterium Aeribacillus pallidus 418. Appl Biochem Biotechnol 171, 31–43 (2013). https://doi.org/10.1007/s12010-013-0348-2

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