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Enhancement of the Enzymatic Digestibility and Ethanol Production from Sugarcane Bagasse by Moderate Temperature-Dilute Ammonia Treatment

Applied Biochemistry and Biotechnology volume 171, pages 1108–1117 (2013)Cite this article

Abstract

In this study, sugarcane bagasse was pretreated with ammonium hydroxide, and the effectiveness of the pretreatment on enzyme hydrolysis and ethanol production was examined. Bagasse was soaked in ammonium hydroxide and water at a ratio of 1:0.5:8 for 0–4 days at 70 °C. Approximately, 14–45 % lignin, 2–6 % cellulose, and 13–22 % hemicellulose were removed during a 0.5- to 4-day ammonia soaking period. The highest glucan conversion of sugarcane bagasse soaked in dilute ammonia at moderate temperature by cellulase was accomplished at 78 % with 75 % of the theoretical ethanol yield. Under the same conditions, untreated bagasse resulted in a cellulose digestibility of 29 and 27 % of the theoretical ethanol yield. The increased enzymatic digestibility and ethanol yields after dilute ammonia pretreatment was related to a combined effect of the removal of lignin and increase in the surface area of fibers.

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Acknowledgments

The present research was conducted by the research fund of Dankook University in 2012.

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Affiliations

  1. Department of Food Science and Nutrition, Dankook University, 126 Jukjeon-Ro, Suji-Gu, Yongin, Gyeonggi-Do, 448-701, Republic of Korea

    Misook Kim

  2. Audubon Sugar Institute, Louisiana State University Agricultural Center, 3845 Highway 75, Saint Gabriel, LA, 70776, USA

    Donal F. Day

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  1. Misook Kim
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  2. Donal F. Day
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Correspondence to Misook Kim.

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Kim, M., Day, D.F. Enhancement of the Enzymatic Digestibility and Ethanol Production from Sugarcane Bagasse by Moderate Temperature-Dilute Ammonia Treatment. Appl Biochem Biotechnol 171, 1108–1117 (2013). https://doi.org/10.1007/s12010-013-0327-7

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  • DOI: https://doi.org/10.1007/s12010-013-0327-7

Keywords

  • Dilute ammonia soaking pretreatment
  • Sugarcane bagasse
  • Moderate temperature
  • Ethanol