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Preparation and Characterization of Polyhydroxyalkanoates Macroporous Scaffold Through Enzyme-Mediated Modifications

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Abstract

Polyhydroxyalkanoates (PHAs) are hydrophobic biodegradable thermoplastics that have received considerable attention in biomedical applications due to their biocompatibility, mechanical properties, and biodegradability. In this study, the degradation rate was regulated by optimizing the interaction of parameters that influence the enzymatic degradation of P(3HB) film using response surface methodology (RSM). The RSM model was experimentally validated yielding a maximum 21 % weight loss, which represents onefold increment in percentage weight loss in comparison with the conventional method. By using the optimized condition, the enzymatic degradation by an extracellular PHA depolymerase from Acidovorax sp. DP5 was studied at 37 °C and pH 9.0 on different types of PHA films with various monomer compositions. Surface modification of scaffold was employed using enzymatic technique to create highly porous scaffold with a large surface to volume ratio, which makes them attractive as potential tissue scaffold in biomedical field. Scanning electron microscopy revealed that the surface of salt-leached films was more porous compared with the solvent-cast films, and hence, increased the degradation rate of salt-leached films. Apparently, enzymatic degradation behaviors of PHA films were determined by several factors such as monomer composition, crystallinity, molecular weight, porosity, and roughness of the surface. The hydrophilicity and water uptake of degraded salt-leached film of P(3HB-co-70%4HB) were enhanced by incorporating chitosan or alginate. Salt-leached technique followed by partial enzymatic degradation would enhance the cell attachment and suitable for biomedical as a scaffold.

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Acknowledgments

The authors wish to thank the USM Fellowship awarded to A. Nor Faezah that has resulted in this article.

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Authors and Affiliations

  1. School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    Nor Faezah Ansari & A. A. Amirul

  2. Malaysian Institute of Pharmaceutical and Nutraceutical, MOSTI, Blok 5-A, 11700, Halaman Bukit Gambir, Penang, Malaysia

    A. A. Amirul

Authors
  1. Nor Faezah Ansari
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  2. A. A. Amirul
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Correspondence to A. A. Amirul.

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Ansari, N.F., Amirul, A.A. Preparation and Characterization of Polyhydroxyalkanoates Macroporous Scaffold Through Enzyme-Mediated Modifications. Appl Biochem Biotechnol 170, 690–709 (2013). https://doi.org/10.1007/s12010-013-0216-0

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  • DOI: https://doi.org/10.1007/s12010-013-0216-0

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