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A Novel Ribonuclease with HIV-1 Reverse Transcriptase Inhibitory Activity from the Edible Mushroom Hygrophorus russula

Applied Biochemistry and Biotechnology volume 170, pages 219–230 (2013)Cite this article

Abstract

A 28-kDa ribonuclease, with an optimum pH of 4.0 and an optimum temperature at 58 °C, was isolated from fruiting bodies of the edible mushroom Hygrophorus russula. It was purified by ion exchange chromatography on carboxymethyl-cellulose, dithyaminoethyl-cellulose, quaternary amine-sepharose and sulphopropyl-sepharose, followed by fast protein liquid chromatography gel filtration on Superdex 75. The N-terminal amino acid sequence was ASAGG which showed homology to those of other fungal RNases to some degree. It exerted the highest RNase activity on poly C and poly U. The Michaelis constant (K m) value of the RNase on yeast tRNA was 3.6 μM, and the maximal velocity (V max) was 0.6 μM. The RNase activity was suppressed by some ions including Fe2+ and Zn2+ ions. The RNase inhibited the activity of HIV-1 reverse transcriptase with an IC50 of 4.64 μM.

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Acknowledgements

This work was financially supported by National Grants of China (Biomass dissociation and low-molecular fragment green monomerization and transformation, 2010CB732202).

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Affiliations

  1. State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, 100193, China

    Mengjuan Zhu, Lijing Xu, Xiao Chen, Zengqiang Ma & Hexiang Wang

  2. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China

    T. B. Ng

Authors
  1. Mengjuan Zhu
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  2. Lijing Xu
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  3. Xiao Chen
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  4. Zengqiang Ma
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  5. Hexiang Wang
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  6. T. B. Ng
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Correspondence to Hexiang Wang or T. B. Ng.

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Zhu, M., Xu, L., Chen, X. et al. A Novel Ribonuclease with HIV-1 Reverse Transcriptase Inhibitory Activity from the Edible Mushroom Hygrophorus russula . Appl Biochem Biotechnol 170, 219–230 (2013). https://doi.org/10.1007/s12010-013-0180-8

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  • DOI: https://doi.org/10.1007/s12010-013-0180-8

Keywords

  • Ribonuclease
  • Purification
  • HIV-1 reverse transcriptase
  • Edible mushroom
  • Hygrophorus russula