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Cell Debris Self-Immobilized Thermophilic Lipase: a Biocatalyst for Synthesizing Aliphatic Polyesters

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Abstract

The paper explored the catalytic activity of a cell debris self-immobilized thermophilic lipase for polyester synthesis, using the ring-opening polymerization of ε-caprolactone as model. Effects of biocatalyst concentration, temperature, and reaction medium on monomer conversion and product molecular weight were systematically evaluated. The biocatalyst displayed high catalytic activity at high temperatures (70–90 °C), with 100 % monomer conversion. High monomer conversion values (>90 %) were achieved in both hydrophobic and hydrophilic solvents, and also in solvent-free system, with the exception of dichloromethane. Poly(ε-caprolactone) was obtained in 100 % monomer conversion, with a number-average molecular weight of 1,680 g/mol and a polydispersity index of 1.35 in cyclohexane at 70 °C for 72 h. Furthermore, the biocatalyst exhibited excellent operational stability, with monomer conversion values exceeding 90 % over the course of 15 batch reactions.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (nos. 21204025, 21074042, and 81102383), the Ministry of Science and Technology of China (International Cooperation and Communication Program 2011DFR51090), Doctoral Fund of Young Scholars of the Ministry of Education (no. 20110061120028), the China Postdoctoral Science Foundation (no. 20110491328), the grant from Jilin Province Science & Technology in China (no. 201101040), and Development and Reform Commission of Jilin Province (no. JF2012C007-3).

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Authors and Affiliations

  1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun, 130012, China

    Yang Sun, Yan Yang, Chenhui Wang, Jiaming Liu, Wei Shi & Quanshun Li

  2. Department of Neurosurgery, the Second Part of First Hospital, Jilin University, Changchun, 130023, China

    Yang Sun & Xiaobo Zhu

  3. Department of Hand Surgery, First Hospital, Jilin University, Changchun, 130023, China

    Laijin Lu

Authors
  1. Yang Sun
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  2. Yan Yang
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  3. Chenhui Wang
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  4. Jiaming Liu
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  5. Wei Shi
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  6. Xiaobo Zhu
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  7. Laijin Lu
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  8. Quanshun Li
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Corresponding authors

Correspondence to Laijin Lu or Quanshun Li.

Additional information

Yang Sun and Yan Yang contributed equally to the work.

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Sun, Y., Yang, Y., Wang, C. et al. Cell Debris Self-Immobilized Thermophilic Lipase: a Biocatalyst for Synthesizing Aliphatic Polyesters. Appl Biochem Biotechnol 170, 399–405 (2013). https://doi.org/10.1007/s12010-013-0152-z

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  • DOI: https://doi.org/10.1007/s12010-013-0152-z

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