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A Natural Isolate Producing Shikimic Acid: Isolation, Identification, and Culture Condition Optimization

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An Erratum to this article was published on 05 May 2013

Abstract

Shikimic acid has wide use in pharmaceuticals due to its application in the synthesis of drug Tamiflu used in the treatment of Swine flu. The high cost and limited availability of shikimic acid isolated from plants has impeded the use of this valuable building block of the drug. In this context, fermentation route to produce shikimic acid from renewable resources has become increasingly attractive. The present study was embarked upon isolation of wild-type microorganisms able to produce shikimic acid. Out of the 42 isolates obtained from the soil, isolate GR-21 was selected as the best with initial production of 0.54 g/L shikimic acid and later identified as Citrobacter sp. The process optimization resulted in 14-fold increase in the shikimic acid production, thereby claiming this process to be a sustainable alternative for the production of this important biomolecule. The process was further scaled up to 14 L bioreactor to validate the production of shikimic acid. Further, the product formed is shikimic acid was confirmed by FTIR analysis. The current studies suggest that the selected isolate could be used as a promising agent to fulfill the worldwide demand of shikimic acid.

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Acknowledgments

We acknowledge Indian Council of Medical Research (ICMR), Govt. of India for financial support to carry out this research work.

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Authors and Affiliations

  1. Department of Microbiology, University of Delhi South Campus, New Delhi, 110021, India

    Garima Rawat, Priyanka Tripathi, Firdaus Jahan & R. K. Saxena

Authors
  1. Garima Rawat
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  2. Priyanka Tripathi
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  3. Firdaus Jahan
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  4. R. K. Saxena
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Corresponding author

Correspondence to R. K. Saxena.

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Fig S1

HPLC of fermentation broth of strain GR-21 after 72h (JPEG 12 kb)

High Resolution Image 1 (TIFF 3269 kb)

Fig. S2

HPLC of the solution obtained from acetic acid washing of the separation column (JPEG 8 kb)

High Resolution Image 2 (TIFF 3162 kb)

Fig. S3

FTIR analysis of purified shikimic acid (JPEG 15 kb)

High Resolution Image 3 (TIFF 3324 kb)

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Rawat, G., Tripathi, P., Jahan, F. et al. A Natural Isolate Producing Shikimic Acid: Isolation, Identification, and Culture Condition Optimization. Appl Biochem Biotechnol 169, 2290–2302 (2013). https://doi.org/10.1007/s12010-013-0150-1

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  • DOI: https://doi.org/10.1007/s12010-013-0150-1

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