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Endopolysaccharides from Ganoderma resinaceum, Phlebia rufa, and Trametes versicolor Affect Differently the Proliferation Rate of HepG2 Cells

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Abstract

Fungi have been used for medicinal purposes for long time by Asian countries, being a putative source of powerful new phytopharmaceuticals such as polysaccharides. The aim of this study was to extract endopolysaccharides (IPS) from Ganoderma resinaceum, Phlebia rufa, and Trametes versicolor, grown under submerged culture, to compare crude IPS production, total carbohydrate, and protein yield, and to study the effect of these IPS on HepG2 cells proliferation rate. Total biomass produced by G. resinaceum, P. rufa, and T. versicolor was (in gram per liter) 3.32 ± 0.80, 5.42 ± 0.58, and 4.2 ± 1.29 and the IPS yield (as the biomass percent) was 9.9 ± 0.05, 29.0 ± 6.3, and 9.1 ± 3.1 %, respectively. Characterization of IPS has shown different proportion between total sugar and protein being, on average 6.04, 10.74, and 22.62, for G. resinaceum, T. versicolor, and P. rufa, respectively. The IPS effect, at 50, 100, and 200 μg mL−1 on HepG2 cell growth and viability was negligible for G. resinaceum and P. rufa but, in the case of T. versicolor, 200 μg mL−1 of IPS evoked 40 % reduction on cell growth. The results suggest that the intracellular polysaccharides from T. versicolor are a potential source for bioactive molecules with anti-proliferative properties.

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Acknowledgments

This work was supported by European Union Funds (FEDER/COMPETE—Operational Competitiveness Programme), by national funds (FCT—Portuguese Foundation for Science and Technology) under the project FCOMP-01-0124-FEDER-022696, and by a research project grant (PEst-C/AGR/UI4033/2011) to AMS, DLS, AAD, and RMB. We acknowledge Professor Carlos Palmeira (University of Coimbra, Portugal) for supplying the HepG2 cells.

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  1. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB-UTAD), University of Trás-os-Montes e Alto Douro, P.O. Box 1013, 5001-801, Vila Real, Portugal

    Amélia M. Silva, Dario L. Santos, Albino A. Dias & Rui M. Bezerra

  2. Department of Biology and Environment, University of Trás-os-Montes e Alto Douro, P.O. Box 1013, 5001-801, Vila Real, Portugal

    Amélia M. Silva, Andreia Miranda, Elisabete Fernandes, Susana Santos, Irene Fraga, Dario L. Santos, Albino A. Dias & Rui M. Bezerra

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  1. Amélia M. Silva
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  2. Andreia Miranda
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  3. Elisabete Fernandes
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  4. Susana Santos
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Correspondence to Amélia M. Silva.

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Silva, A.M., Miranda, A., Fernandes, E. et al. Endopolysaccharides from Ganoderma resinaceum, Phlebia rufa, and Trametes versicolor Affect Differently the Proliferation Rate of HepG2 Cells. Appl Biochem Biotechnol 169, 1919–1926 (2013). https://doi.org/10.1007/s12010-013-0107-4

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