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Selection of DNA Aptamers with Affinity for Pro-gastrin-Releasing Peptide (proGRP), a Tumor Marker for Small Cell Lung Cancer

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Abstract

Aptamers are single-strand oligonucleotides that are generated by the systemic evolution of ligands by exponential enrichment (SELEX) technique and that can bind to target molecules specifically. However, only a few aptamers have been developed to date against tumor markers. To utilize aptamers for tumor diagnosis, a variety of aptamers are required. Here, a single-stranded DNA aptamer specific for pro-gastrin-releasing peptide (proGRP), a marker for small cell lung cancer, was selected using SELEX. After selection, identical sequences were found in the DNA library. This sequence was selected and its binding affinity to proGRP was evaluated using surface plasmon resonance.

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Acknowledgments

Part of this work was supported by the JSPS International Training Program.

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Authors and Affiliations

  1. Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259, Nagatsuta, Midori-ku, Yokohama, Kanagawa, Japan

    Masayasu Mie, Tetsuro Kai & Eiry Kobatake

  2. Department of Chemistry and Institute of Biomedical Engineering, Imperial College, London, UK

    Thao Le & Anthony E. G. Cass

Authors
  1. Masayasu Mie
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  2. Tetsuro Kai
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  3. Thao Le
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  4. Anthony E. G. Cass
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  5. Eiry Kobatake
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Correspondence to Eiry Kobatake.

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Mie, M., Kai, T., Le, T. et al. Selection of DNA Aptamers with Affinity for Pro-gastrin-Releasing Peptide (proGRP), a Tumor Marker for Small Cell Lung Cancer. Appl Biochem Biotechnol 169, 250–255 (2013). https://doi.org/10.1007/s12010-012-9956-5

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  • DOI: https://doi.org/10.1007/s12010-012-9956-5

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