Applied Biochemistry and Biotechnology

, Volume 169, Issue 1, pp 88–99 | Cite as

Control of the Harmful Alga Microcystis aeruginosa and Absorption of Nitrogen and Phosphorus by Candida utilis

Article

Abstract

This study is aimed at controlling eutrophication through converting the nutrients such as nitrogen and phosphorus into microbial protein and simultaneously inhibiting the growth of Microcystis aeruginosa by Candida utilis. C. utilis and M. aeruginosa (initial cell density was 2.25 × 107 and 4.15 × 107 cells·mL−1) were cultured together in the absence or presence of a carbon source (glucose) during a 10-day experiment. In the absence of carbon source, the measured removal efficiencies of NH4+–N and PO43−–P were 41.39 ± 2.19 % and 82.93 ± 3.95 %, respectively, at the second day, with the removal efficiency of 67.82 ± 2.29 % for M. aeruginosa at the fourth day. In contrast, the removal efficiencies of NH4+–N and PO43−–P were increased to 87.45 ± 4.25 % and 83.73 ± 3.55 %, respectively, while the removal efficiency of M. aeruginosa decreased to 37.89 ± 8.41 % in the presence of the carbon source (C/N = 2:1). These results showed that the growth of M. aeruginosa was inhibited by C. utilis. Our finding sheds light on a novel potential approach for yeast to consume nutrients and control harmful algal during bloom events.

Keywords

Eutrophication Biological control Candida utilis Microcystis aeruginosa Competition 

References

  1. 1.
    APHA. (1998). Standard Methods for the Examination of Water and Wastewater (20th ed.). Washington, DC: American Public Health Association (APHA).Google Scholar
  2. 2.
    Arnold, J. L., Knapp, J. S., & Johnson, C. L. (2000). Water Research, 34, 3699–3708.CrossRefGoogle Scholar
  3. 3.
    Auesukaree, C., Homma, T., Tochio, H., Shirakawa, M., Kaneko, Y., & Harashima, S. (2004). Journal of Biological Chemistry, 279, 17289–17294.CrossRefGoogle Scholar
  4. 4.
    Avnimelech, Y. (1999). Aquaculture, 176, 227–235.CrossRefGoogle Scholar
  5. 5.
    Chang, H.-Q., Yang, X.-E., Fang, Y.-Y., Pu, P.-M., Li, Z.-K., & Rengel, Z. (2006). Journal of Zhejiang University. Science. B, 7, 521–531.CrossRefGoogle Scholar
  6. 6.
    Conley, D. J., Paerl, H. W., Howarth, R. W., Boesch, D. F., Seitzinger, S. P., Havens, K. E., et al. (2009). Science, 323, 1014–1015.CrossRefGoogle Scholar
  7. 7.
    Davis, J. R., & Koop, K. (2006). Hydrobiologia, 559, 23–76.CrossRefGoogle Scholar
  8. 8.
    Figueiredo, D. R., Azeiteiro, U. M., Esteves, S. M., Goncalves, F. J. M., & Pereira, M. J. (2004). Ecotoxicology and Environmental Safety, 59, 151–163.CrossRefGoogle Scholar
  9. 9.
    Figueredo, C. C., & Giani, A. (2001). Hydrobiologia, 445, 165–174.CrossRefGoogle Scholar
  10. 10.
    Gao, G., Qin, B. Q., Sommaruga, R., & Psenner, R. (2007). Hydrobiologia, 581, 177–188.CrossRefGoogle Scholar
  11. 11.
    Hansson, L. A., Annadotter, H., Bergman, E., Hamrin, S. F., Jeppesen, E., Kairesalo, T., et al. (1998). Ecosystems, 1, 558–574.CrossRefGoogle Scholar
  12. 12.
    Huett, D. O., Morris, S. G., Smith, G., & Hunt, N. (2005). Water Research, 39, 3259–3272.CrossRefGoogle Scholar
  13. 13.
    Ikawa, M., Sasner, J. J., & Haney, J. F. (2001). Hydrobiologia, 443, 19–22.CrossRefGoogle Scholar
  14. 14.
    Jeong, H. J., Kim, J. S., Yoo, Y. D., Kim, S. T., Song, J. Y., Kim, T. H., et al. (2008). Harmful Algae, 7, 368–377.CrossRefGoogle Scholar
  15. 15.
    Ke, Z. X., Xie, P., Guo, L. G., Liu, Y. Q., & Yang, H. (2007). Aquaculture, 265, 127–138.CrossRefGoogle Scholar
  16. 16.
    Li, M., Wu, Y. J., Yu, Z. L., Sheng, G. P., & Yu, H. Q. (2007). Water Research, 41, 3152–3158.CrossRefGoogle Scholar
  17. 17.
    Marazioti, C., Kornaros, M., & Lyberatos, G. (2003). Water Research, 37, 1239–1251.CrossRefGoogle Scholar
  18. 18.
    Oberholster, P. J., Botha, A. M., & Ashton, P. J. (2009). Ecotoxicology, 18, 34–46.CrossRefGoogle Scholar
  19. 19.
    Pitois, S., Jackson, M. H., & Wood, B. J. B. (2001). Journal of Environmental Health, 64, 25–32.Google Scholar
  20. 20.
    Qin, B. Q., Yang, L. Y., Chen, F. Z., Zhu, G. W., Zhang, L., & Chen, Y. Y. (2006). Chinese Science Bulletin, 51, 2401–2412.CrossRefGoogle Scholar
  21. 21.
    Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M., & Stanier, R. Y. (1979). Journal of General Microbiology, 111, 1–61.CrossRefGoogle Scholar
  22. 22.
    Singhal, V., & Rai, J. P. N. (2003). Bioresource Technology, 86, 221–225.CrossRefGoogle Scholar
  23. 23.
    Smith, V. H., Tilman, G. D., & Nekola, J. C. (1999). Environmental Pollution, 100, 179–196.CrossRefGoogle Scholar
  24. 24.
    Sondergaard, M., Liboriussen, L., Pedersen, A. R., & Jeppesen, E. (2008). Ecosystems, 11, 1291–1305.CrossRefGoogle Scholar
  25. 25.
    Song, L. R., Chen, W., Peng, L., Wan, N., Gan, N. Q., & Zhang, X. M. (2007). Water Research, 41, 2853–2864.CrossRefGoogle Scholar
  26. 26.
    Tucker, S., & Pollard, P. (2005). Applied and Environmental Microbiology, 71, 629–635.CrossRefGoogle Scholar
  27. 27.
    Tang, X. Q., Wu, M., Yang, W. J., Yin, W., Jin, F., Ye, M., et al. (2012). Water, Air, and Soil Pollution, 223, 723–737.CrossRefGoogle Scholar
  28. 28.
    Watanabe, T., Masaki, K., Iwashita, K., Fujii, T., & Iefuji, H. (2009). Bioresource Technology, 100, 1781–1785.CrossRefGoogle Scholar
  29. 29.
    Watanabe, T., Ozaki, N., Iwashita, K., Fujii, T., & Iefuji, H. (2008). Applied Microbiology and Biotechnology, 80, 331–338.CrossRefGoogle Scholar
  30. 30.
    White, S. H., Duivenvoorden, L. J., & Fabbro, L. D. (2005). Hydrobiologia, 548, 117–126.CrossRefGoogle Scholar
  31. 31.
    Wicks, R. J., & Thiel, P. G. (1990). Environmental Science & Technology, 24, 1413–1418.CrossRefGoogle Scholar
  32. 32.
    Yang, X. E., Wu, X., Hao, H. L., & He, Z. L. (2008). Journal of Zhejiang University. Science. B, 9, 197–209.CrossRefGoogle Scholar
  33. 33.
    Yoshida, T., Takashima, Y., Tomaru, Y., Shirai, Y., Takao, Y., Hiroishi, S., et al. (2006). Applied and Environmental Microbiology, 72, 1239–1247.CrossRefGoogle Scholar
  34. 34.
    Zhang, X., Hu, H. Y., Men, Y. J., Yang, J., & Christoffersen, K. (2009). Water Research, 43, 2953–2960.CrossRefGoogle Scholar
  35. 35.
    Zhu, L., Ding, W., Feng, L. J., Kong, Y., Xu, J., & Xu, X. Y. (2012). Bioresource Technology, 108, 1–7.CrossRefGoogle Scholar
  36. 36.
    Zohary, T. (1985). Journal of Plankton Research, 7, 399–409.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Yun Kong
    • 1
    • 2
  • Xiangyang Xu
    • 2
  • Liang Zhu
    • 2
  • Lihong Miao
    • 1
  1. 1.School of Biology and Pharmaceutical EngineeringWuhan Polytechnic UniversityWuhanPeople’s Republic of China
  2. 2.Department of Environmental EngineeringZhejiang UniversityHangzhouPeople’s Republic of China

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