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Isolation and Characterization of Pseudomonas sp. STM 997 from Soil Sample having Potentiality to Degrade 3,6-Dimethyl-1-keto-1,2,3,4-tetrahydrocarbazole: A Novel Approach

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Abstract

A pure colony of a bacterium from contaminated soil was isolated by exploiting 3,6-dimethyl-1-keto-1,2,3,4–tetrahydrocarbazole, a novel carbazole derivative, having indole moiety as well as 3-methyl functionality both in aromatic and hydro-aromatic moiety, as a sole source of carbon and energy. Taxonomical studies, biochemical analysis, and 16S rDNA sequence analysis indicated that the isolated strain has close similarity with Pseudomonas sp. Thin-layer chromatography followed by HPLC and mass spectroscopic study indicates that the isolated Pseudomonas sp. STM 997 degrades 3,6-dimethyl-1-keto-1,2,3,4–tetrahydrocarbazole, and this strain may be useful in the bioremediation of environments contaminated by the compounds containing carbazole moiety with methyl substituents at various reactive sites. This study also provides an evidence in favor of the suggested biodegradation of 3-methylcarbazole to carbazole in plants.

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Acknowledgments

The authors are thankful to Prof. Krishnangshu Roy, Director, Prof. Tamal Ghosh, MSVP, and Prof. Santanu Tripathi, Head of the Department of Clinical & Experimental Pharmacology, School of Tropical Medicine, Kolkata, for their interest in the work. The authors would like to acknowledge Dr. Sanjoy Ghosh, Department of Biochemistry, University of Calcutta for helpful suggestions. We are also thankful to the Council of Scientific and Industrial Research (CSIR), New Delhi, India for providing a Senior Research Fellowship (grant no. 09/951(0001)/2008-EMR-I) to one (SC) of the authors.

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Authors and Affiliations

  1. Calcutta School of Tropical Medicine, Kolkata, India

    Biswanath Chakraborty, Suchandra Chakraborty, Anjan Kumar Basu & Chandan Saha

  2. White Line Export Pvt. Ltd., Kolkata, India

    Bhrigu Aditya

  3. Bose Institute, Kolkata, India

    T. P. Sinha & Tanima Modak Dhar

  4. Department of Clinical and Expt. Pharmacology, Calcutta School of Tropical Medicine, Kolkata, India

    Chandan Saha

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  1. Biswanath Chakraborty
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  2. Suchandra Chakraborty
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  3. Anjan Kumar Basu
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  4. Bhrigu Aditya
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  5. T. P. Sinha
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  6. Tanima Modak Dhar
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  7. Chandan Saha
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Correspondence to Chandan Saha.

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Chakraborty, B., Chakraborty, S., Basu, A.K. et al. Isolation and Characterization of Pseudomonas sp. STM 997 from Soil Sample having Potentiality to Degrade 3,6-Dimethyl-1-keto-1,2,3,4-tetrahydrocarbazole: A Novel Approach. Appl Biochem Biotechnol 168, 1765–1777 (2012). https://doi.org/10.1007/s12010-012-9895-1

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  • DOI: https://doi.org/10.1007/s12010-012-9895-1

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