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Kinetic Studies on the Product Inhibition of Enzymatic Lignocellulose Hydrolysis

Applied Biochemistry and Biotechnology volume 167, pages 358–366 (2012)Cite this article

Abstract

In order to understand the product inhibition of enzymatic lignocellulose hydrolysis, the enzymatic hydrolysis of pretreated rice straw was carried out over an enzyme loading range of 2 to 30 FPU/g substrate, and the inhibition of enzymatic hydrolysis was analyzed kinetically based on the reducing sugars produced. It was shown that glucose, xylose, and arabinose were the main reducing sugar components contained in the hydrolysate. The mass ratio of glucose, xylose, and arabinose to the total reducing sugars was almost constant at 52.0 %, 29.7 % and 18.8 %, respectively, in the enzyme loading range. The reducing sugars exerted competitive inhibitory interferences to the enzymatic hydrolysis. Glucose, xylose, and arabinose had a dissociation constant of 1.24, 0.54 and 0.33 g/l, respectively. The inhibitory interferences by reducing sugars were superimposed on the enzymatic hydrolysis. The enzymatic hydrolysis could be improved by the removal of the produced reducing sugars from hydrolysate.

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Acknowledgments

This research project was financially supported by the grants of the National Natural Science Foundation of China and the People’s Government of Guangdong Province (U0733001), and the National Basic Research Program of China (973 Program, 2009CB724700).

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Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industrial Technology, Nanjing University of Technology, Nanjing, Jiangsu, 210009, China

    Yelian Miao, Xuejian Jiang & Zhou Huang

  2. Faculty of Bioresource Science, Akita Prefectural University, Akita, Akita, 010-0195, Japan

    Jie Yu Chen

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  1. Yelian Miao
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  2. Jie Yu Chen
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  4. Zhou Huang
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Correspondence to Yelian Miao.

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Miao, Y., Chen, J.Y., Jiang, X. et al. Kinetic Studies on the Product Inhibition of Enzymatic Lignocellulose Hydrolysis. Appl Biochem Biotechnol 167, 358–366 (2012). https://doi.org/10.1007/s12010-012-9689-5

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  • DOI: https://doi.org/10.1007/s12010-012-9689-5

Keywords

  • Biomass
  • Enzymatic hydrolysis
  • Saccharification
  • Product inhibition
  • Kinetics