Skip to main content

Advertisement

SpringerLink
Log in

Assessment of Dentifrices Against Candida Biofilm

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The invasion of opportunistic pleiomorphic Candida albicans into oral cavity environment leads to development and progression of its resistance to both naturally occurring antifungal peptides in human saliva as well as commercially available antifungal therapies. As a result of this, the usage and popularity of natural medicine and dentifrices had increased significantly in the last decade. In the present investigation, we have assessed the action of locally available dentifrices against C. albicans biofilm. Disk diffusion test showed maximum zone of inhibition (20 mm) by herbal dentifrice (D-5) as compared to other dentifrices when incubated at 37 °C and 48 h. Assessment of dentifrice D-5 for its effectiveness against C. albicans was further shown in MIC90 (3.12 mg mL−1) and SMIC90 (6.2 mg mL−1) values for planktonic and sessile cells (biofilm forming), respectively. Our data depicted 80% reduction in the cell surface hydrophobicity when 6.2 mg mL−1 of herbal dentifrice D-5 was used against 48-h grown Candida biofilm at 37 °C. Visualization of herbal dentifrice D-5-treated C. albicans biofilm under SEM revealed drastic reduction in the dense network of yeast, hyphae, and pseudohyphae enclosed in its ECM as compared to its control biofilm. The data were further supported by CLSM analysis which depicted C. albicans architecture disruption by herbal dentifrices. From the above data, it is inferred that these studies would provide researchers and medical practitioners with better insight into the antifungal effect of natural herbal dentifrices.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Samaranayake, L. P., Keung Leung, W., & Jin, L. (2009). Periodontology, 49, 39–59.

    Article  Google Scholar 

  2. Samaranayake, L. P., Cheung, L. K., & Samaranayake, Y. H. (2002). Dermatologic Therapy, 15, 251–269.

    Article  Google Scholar 

  3. Coogan, M. M., Fidel, P. L., Jr., Komesu, M. C., Maeda, N., & Samaranayake, L. P. (2006). Advances in Dental Research, 19, 130–138.

    Article  CAS  Google Scholar 

  4. Luque, A. G., Biasoli, M. S., Tosello, M. E., Binolfi, A., Lupo, S., & Magaro, H. M. (2009). Mycoses, 52, 53–59.

    Article  CAS  Google Scholar 

  5. Wei, G. X., Xu, X., & Wu, C. D. (2011). Archives of Oral Biology, 56, 565–572.

    Article  CAS  Google Scholar 

  6. Douglas, L. J. (2003). Trends in Microbiology, 11, 30–36.

    Article  CAS  Google Scholar 

  7. Ruhnke, M. (2006). Current Drug Targets, 74, 495–504.

    Article  Google Scholar 

  8. Nett, J. E., Guite, K. M., Ringeisen, A., Holoyda, K. A., & Andes, D. R. (2008). Antimicrobial Agents and Chemotherapy, 52, 3411–3413.

    Article  CAS  Google Scholar 

  9. Uppuluri, P., Chaturvedi, A. K., Srinivasan, A., Banerjee, M., Ramasubramaniam, A. K., Köhler, J. R., Kadosh, D., & Lopez-Ribot, J. L. (2010). Dispersion as an important step in the Candida albicans biofilm developmental cycle. PLoS Pathogens, 6(3), e1000828.

    Article  Google Scholar 

  10. Samaranayake, Y. H., Ye, J., Yau, J. Y. Y., Cheng, B. P. K., & Samaranayakae, L. P. (2004). Journal of Clinical Microbiology, 43, 818–825.

    Article  Google Scholar 

  11. Marsh, P., & Martin, M. V. (1999). Oral Microbiology (4th ed.). Oxford: England.

    Google Scholar 

  12. Arendorf, T. M., & Walker, D. M. (1980). Archives of Oral Biology, 25, 1–10.

    Article  CAS  Google Scholar 

  13. Jacob, L. S., Flaitz, C. M., Nichols, C. M., & Hicks, M. J. (1998). Journal of the American Dental Association, 129, 187–194.

    CAS  Google Scholar 

  14. Hodson, J. J., & Craig, G. T. (1972). The Dental Practitioner and Dental Record, 22, 296–301.

    CAS  Google Scholar 

  15. Lynch, E., & Beighton, D. A. (1994). Caries Research, 28, 233–239.

    Article  CAS  Google Scholar 

  16. Slots, J., Rams, T. E., & Listgarten, M. A. (1988). Oral Microbiology and Immunology, 3, 47–52.

    Article  CAS  Google Scholar 

  17. Zambon, J. J., Reynolds, H. S., & Genco, R. J. (1990). Journal of Periodontology, 61, 699–704.

    Article  CAS  Google Scholar 

  18. Pizzo, G., Barchiesi, F., Falconi, D., Francesco, L., Giuliana, G., & Arzeni, D. (2002). Archives of Oral Biology, 47, 189–196.

    Article  CAS  Google Scholar 

  19. Reynaud, A. H., Nygaard-Ostby, B., Boygard, G. K., Eribe, E. R., Olsen, I., & Gjermo, P. (2001). Journal of Clinical Periodontology, 28, 860–864.

    Article  CAS  Google Scholar 

  20. Siqueira, J. F., & Sen, B. H. (2004). Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 97, 632–641.

    Article  Google Scholar 

  21. Kumamoto, C. A., & Vinces, M. D. (2005). Annual Review of Microbiology, 59, 113–133.

    Article  CAS  Google Scholar 

  22. Dolan, R. M. (2001). Emerging Infectious Diseases, 7, 277–281.

    Article  Google Scholar 

  23. Lal, P., Sharma, D., Pruthi, P., & Pruthi, V. (2010). Journal of Applied Microbiology, 109, 128–136.

    Google Scholar 

  24. Chandra, J., Kuhn, D. M., Mukhrjee, P. K., Hoyer, L. L., McCormick, T., & Ghannoum, M. A. (2001). Journal of Bacteriology, 183, 5385–5394.

    Article  CAS  Google Scholar 

  25. Agarwal, V., Lal, P., & Pruthi, V. (2008). Mycopathologia, 165, 13–19.

    Article  CAS  Google Scholar 

  26. Zhang, A. Y., Camp, W. L., & Elewski, B. E. (2007). Dermatologic Clinics, 25, 165–183.

    Article  CAS  Google Scholar 

  27. Jain, N., Kohli, R., Cook, E., Gialanella, P., Chang, T., & Fries, B. C. (2007). Applied and Environmental Microbiology, 73, 1697–1703.

    Article  CAS  Google Scholar 

  28. Meyer, B. (2003). International Biodeterioration & Biodegradation, 51, 249–253.

    Article  CAS  Google Scholar 

  29. Bruzual, I., Riggle, P., Hadley, S., & Kumamoto, C. A. (2007). Journal of Antimicrobial Chemotherapy, 59, 441–450.

    Article  CAS  Google Scholar 

  30. Sheehan, D. J., Hitchcock, C. A., & Sibley, C. M. (1999). Clinical Microbiology Reviews, 12, 40–79.

    CAS  Google Scholar 

  31. Sanglard, D., Ischer, F., Parkinson, T., Falconer, D., & Bille, J. (2003). Antimicrobial Agents and Chemotherapy, 47, 2404–2412.

    Article  CAS  Google Scholar 

  32. Okpalugo, J., Ibrahim, K., & Inyang, U. S. (2009). Tropical Journal of Pharmaceutical Research, 8, 71–77.

    Article  Google Scholar 

  33. Lee, S. S., Zhang, W., & Li, Y. (2004). Journal of the American Dental Association, 135, 1133–1141.

    Google Scholar 

  34. Poureslami, H. R., Makarem, A., & Mojab, F. (2007). Journal of Dental Research, 4, 106–110.

    Google Scholar 

  35. Mohire, N. C., & Yadav, A. V. (2010). Indian Journal of Dental Research, 21, 380–384.

    Article  Google Scholar 

  36. Patil, S., Venkataraghavan, K., Anantharaj, A., Patil, S. (2010). International Dentistry SA. 12, 72–82.

  37. Yigit, N., Aktas, E., & Ayyildiz, A. (2008). Journal of Medical Mycology, 18, 141–146.

    Google Scholar 

  38. Radafshar, G., Mahboob, F., & Kazemnejad, E. (2010). Journal of Medicinal Plants Research, 4, 1182–1186.

    Google Scholar 

  39. Barrett, B., Kiefer, D., & Rabago, D. (1999). Alternative Therapies in Health and Medicine, 5, 40–49.

    CAS  Google Scholar 

  40. Eisenberg, D. M., Davis, R. B., & Ettner, S. L. (1998). Journal of the American Medical Association, 280, 1569–1575.

    Article  CAS  Google Scholar 

  41. Thibault, C. (2001). Probe, 35, 25–28.

    Google Scholar 

  42. Mullally, B. H., James, J. A., Coulter, W. A., & Linden, G. J. (1995). Journal of Clinical Periodontology, 22, 686–689.

    Article  CAS  Google Scholar 

  43. Preston, A. J. (1998). Dental Update, 25, 247–253.

    CAS  Google Scholar 

  44. Silva, W. J., Seneviratne, J., Parahitiyawa, N., Rosa, E. A., Samaranayake, L. P., & Cury, A. A. (2008). Brazilian Dental Journal, 19, 364–369.

    Google Scholar 

  45. Klotz, S. A., Drutz, D. J., & Zajic, J. E. (1985). Infection and Immunity, 50, 97–101.

    CAS  Google Scholar 

  46. de Freitas Fernandes, F. S., Pereira-Cenci, T., da Silva, W. J., Filho, A. P. R., Straioto, F. G., & Del Bel Cury, A. A. (2010). Journal of Prosthetic Dentistry, 105, 51–58.

    Article  Google Scholar 

  47. Binney, A., Addy, M., McKeown, S., & Everatt, L. (1995). Journal of Clinical Periodontology, 22, 830–834.

    Article  CAS  Google Scholar 

  48. Mankodi, S., Bartizek, R. D., Winston, J. L., Biesbrock, A. R., McClanahan, S. F., & He, T. (2005). Journal of Clinical Periodontology, 32, 75–80.

    Article  CAS  Google Scholar 

  49. Moran, J., Newcombe, R. G., Wright, P., Haywood, J., Marlow, I., & Addy, M. (2005). Journal of Clinical Periodontology, 32, 841–845.

    Article  CAS  Google Scholar 

  50. Müller, H. P., Barrieshi-Nusair, K. M., Könönen, E., & Yang, M. (2006). Journal of Clinical Periodontology, 33, 811–818.

    Article  Google Scholar 

  51. Ellepola, A. N. B., Khan, Z. U., Chandy, R., & Philip, L. (2011). Medical Principles and Practice, 20, 112–117.

    Article  Google Scholar 

  52. Cao, X. H., Liao, Z. Y., Wang, C. L., Yang, W. Y., & Lu, M. F. (2009). Brazilian Journal of Microbiology, 40, 373–379.

    Article  CAS  Google Scholar 

  53. Borecka-Melkusova, S., & Bujadakova, H. (2008). Canadian Journal of Microbiology, 54, 718–724.

    Article  CAS  Google Scholar 

  54. Silva, T. M., Glee, P. M., & Hazen, K. C. (1995). Journal of Medical and Veterinary Mycology, 33, 117–122.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, IIT Roorkee, Roorkee, 247667, Uttarakhand, India

    Nivedita Singh, Akhansha Nayyar & Vikas Pruthi

  2. Department of Chemistry, IIT Roorkee, Roorkee, 247667, Uttarakhand, India

    Nivedita Singh, G. Bhattacharjee & A. K. Singh

Authors
  1. Nivedita Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akhansha Nayyar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Bhattacharjee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. K. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Vikas Pruthi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vikas Pruthi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singh, N., Nayyar, A., Bhattacharjee, G. et al. Assessment of Dentifrices Against Candida Biofilm. Appl Biochem Biotechnol 167, 1688–1698 (2012). https://doi.org/10.1007/s12010-012-9574-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-9574-2

Keywords

Search

Navigation